Last month Nature published a paper that revealed what many scientists knew or suspected. Cancer cell lines evolve very quickly due to positive clonal selection driven by cell culture conditions. And that this is likely the reason why the same cell line used in different labs […]
An interesting round up of the Top 10 Sequencing Companies from Genetic Engineering & Biotechnology News highlights the dominance of Illumina on the life-sciences (genomics) industry. Illumina’s $2.752 billion in 2017 is almost double the combined revenue of the other 9 companies ($1.445 billion). With giants […]
Last night I watched in amazement as a beating heart was brought into the UK for transplant surgery – the heart was kept alive in the OCS HEART system. The BBC program Heart Transplant: A Chance to Live is outstanding TV and if you can […]
There’s a great paper from Pete Skene, postdoc 2011-2017 in the Henikoff lab, on eLife: An efficient targeted nuclease strategy for high-resolution mapping of DNA binding sites. The paper describes a novel method that performs as well as ChIP-Seq, but with lower background and false-positives. ChIP-seq is […]
I wanted to let you readers of my blog that a huge change is coming for me. At the end of February 2018 I’ll be leaving my job here at CRUK and am joining Astra Zeneca as the Director of a new R&D group. My time at CRUK […]
Because HPV has such a small and relatively simple genome (8,000 bp encoding 8 genes) it is possible to screen for genetic variation that may underly carcinogenesis. A team from the National Cancer Institute (NCI) has just published the sequencing of over 5,570 human cell and tissue samples – […]
Hopefully this post is going to pop up as if by magic to all my LinkedIn contacts. This is something I’ve been meaning to do for ages and finally had a go. Thanks to the people at WPBeginner for writing this “how to guide” to make […]
Prof Yutaka Suzuki’s lab at the University of Tokyo published a paper last week in DNA research: Sequencing and phasing cancer mutations in lung cancers using a long-read portable sequencer. In the paper they described their work detecting SNVs in EGFR, KRAS, NRAS and NF1, and EML4-ALK, […]
Iain Macaulay organised a #singlecell meeting at Norwich’s Earlham Institute earlier this month. The meeting was fun and there were some great presentations during a day of talks and discussion on the development and application of single cell technologies. 100+ #singlecell analysis methods at www.tinyurl.com/mqabyur One that […]
A paper uploaded to BioRxiv yesterday is the first (almost) published report of index-swapping on Illumina’s Exclusion-Amplification (ExAmp) chemistry. The paper: Index Switching Causes “Spreading-Of-Signal” Among Multiplexed Samples In Illumina HiSeq 4000 DNA Sequencing, by a group from Stanford Functional Genomics Facility published a re-analysis of single-cell […]
