Iain Macaulay organised a #singlecell meeting at Norwich’s Earlham Institute earlier this month. The meeting was fun and there were some great presentations during a day of talks and discussion on the development and application of single cell technologies. 100+ #singlecell analysis methods at www.tinyurl.com/mqabyur One that […]
A paper uploaded to BioRxiv yesterday is the first (almost) published report of index-swapping on Illumina’s Exclusion-Amplification (ExAmp) chemistry. The paper: Index Switching Causes “Spreading-Of-Signal” Among Multiplexed Samples In Illumina HiSeq 4000 DNA Sequencing, by a group from Stanford Functional Genomics Facility published a re-analysis of single-cell […]
Confession: this post may not actually make your 10X Genomics experiments ten times cheaper…but implement Demuxlet in your workflow and with the right sort of experiment you might just get there! Single-cell RNA-Seq might just have got a whole lot cheaper, thanks to a method […]
The Oxford Nanopore MinION is generating some truly staggering read-lengths. Keith Robison has summarised many of the major advances of the past few months; MinKNOW increasing yield by reducing pore blockage with a “nano-Heimlich manoeuvre” which increases yields to 10Gb (ONT’s CSO Clive Brown has self […]
I’m not in tomorrow as I’m off to a funeral so I’ll not be writing anymore about the new sequencer from Illumina till later in the week. But here’s my initial thoughts… Illumina’s presentation at JP Morgan is over and they’ve certainly delivered. NovaSeq is the latest […]
Merry Christmas to everyone who’s read this blog last year. I hope you are looking forward to 2017 as much as I, there’s some cool stuff coming in 2017. James.
I’m predicting that 2017 will be the breakthrough year for nanopore sequencing. Having seen what’s been going on over the last 12-24 months I’m pretty confident that we’ll see at least one NGS niche being taken over by Nanopore sequencing in 2017. Whether this will […]
A couple of Tweets today point to the amazing lengths Oxford Nanopores MinION sequencer is capable of generating – over 400kb! Dominik Handler Tweeted a plot showing read distribution from a run . In replies following the Tweet he describes the DNA handling as […]
The UK recently approved an NIPT test to screen high risk pregnancies for foetal trisomy 21, 13, or 18 after the current primary screening test, and in place of amniocentesis (following on from the results of the RAPID study). I am 100% in favour of […]
DeciBio have a great interactive Tableau dashboard which you can use to browse and filter their analysis of 97 “laboratory biomarker analysis†ImmunOncolgy clinical trials; see: Diagnostic Biomarkers for Cancer Immunotherapy – Moving Beyond PD-L1. The raw data comes from ClinicalTrials.gov where you can specify […]
