24 11, 2017

SPLiT-Seq: single-cell RNA-Seq without the hardware

By | November 24th, 2017|Categories: Methods and applications, Next-generation sequencing, Single-cell sequencing|Tags: , , |1 Comment

I’ve been meaning to write up a post on a BioRxiv report from earlier this year: “Scaling single cell transcriptomics through split pool barcoding”1. The Seelig Lab at the University of Washington have developed a single-cell RNA sequencing method to enable labelling RNA molecules with cell-of-origin information using […]

2 03, 2017

APOBEC-Seq for DNA methylation without the bisuflite

By | March 2nd, 2017|Categories: Methods and applications, Next-generation sequencing|Tags: , |1 Comment

For me one of the top talks at #AGBT17 was in a concurrent session. Theodore Davis from New England Biolabs presented “APOBEC-Seq: An enzymatic method for methylome analysis at single-base resolution without bisulfite treatment.” His talk focused on some of the cons of using sodium bisulfite sequencing for methylation in epigenetic […]

14 02, 2017

Understanding a 10XGenomics Cell ranger QC report

By | February 14th, 2017|Categories: "Experimental design controls etc", Single-cell sequencing|Tags: , |5 Comments

This post: “Understanding your 10XGenomics cell ranger report” originally appeared on my labs group blog but I thought that people working with single-cell who are at AGBT might like to read it too. Understanding your @10Xgenomics cell ranger reports The 10X Genomics Cell Ranger analysis […]

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