22 02, 2017

Hydroxymethylation (hmC) papers from CEGX

By | February 22nd, 2017|Categories: Methods and applications, Next-generation sequencing|Tags: , , |0 Comments

Cambridge Epigenetix (@CEGX_news) have a list of almost 50 papers on their website using their technology for DNA methylation and hydroxymethylation analysis, which I thought readers of CoreGenomics might be interested in. They also have a list of Epigenetics Conferences coming up….enjoy! The papers: Chen et […]

15 02, 2017

Nanostring Hyb&Seq

By | February 15th, 2017|Categories: Conferences, Methods and applications, Next-generation sequencing, Other stuff|Tags: , , |4 Comments

Nanostring had an interesting poster presented by Joe Beecham at last years AGBT describing their novel single-molecule hybridization-based sequencing chemistry (see AGBT and JPMorgan 2016 coverage from GenomeWeb). This utilizes the Nanostring optical barcodes in a new configuration to deliver Hyb&Seq: no amplification, no enzymes, and no […]

10 02, 2017

Fingerprinting for multiplexed single-cell RNA-seq

By | February 10th, 2017|Categories: "Experimental design controls etc", "My almost"..., 10X Genomics, Methods and applications, Next-generation sequencing, Single-cell sequencing|Tags: |0 Comments

In this post I describe an idea for using SNP fingerprinting for single-cell RNA-Seq to identify which sample each individual cell comes from in a multiplexed library prep. The reason for my thinking about this is that single-cell experiments are expensive. Although the cost-per-cell can […]

24 03, 2014

5mC-PCR: preserving methylation status during polymerase chain reaction

By | March 24th, 2014|Categories: Methods and applications|6 Comments

Methylation analysis is hampered by the simple fact that PCR amplification removes methylation marks from native DNA. We came up with a simple idea to produce a thermo-stable DNA methyltransferase to preserve methylation status through PCR cycles, allowing amplification of DNA and simplified analysis. The […]

29 08, 2013

Targeted RNA-seq methods are here

By | August 29th, 2013|Categories: Exomes and amplicons, Methods and applications, Next-generation sequencing|2 Comments

Illumina and Life Technologies are both launching targeted RNA-seq applications which are likely to become standard tools for many labs; if the price is right. The ability to target a portion of the genome has revolutionised next-generation sequencing experiments. The analysis of exomes has exploded, […]

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