8 06, 2018

Single-cell indexing for methylation analysis (sci-MET)

By | June 8th, 2018|Categories: Library Prep, Methods and applications, Single-cell sequencing|0 Comments

Andrew Adey’s group at OHSU published sci-MET – a single cell method for whole-genome methylation analysis in Nature Biotech last month: Highly scalable generation of DNA methylation profiles in single cells. In this they described their indexing method and report on an analysis of over 3000 […]

17 05, 2018

Size matters…for cfDNA (and ctDNA)

By | May 17th, 2018|Categories: ctDNA, Library Prep, Methods and applications, Next-generation sequencing|0 Comments

“It’s not the size that matters, it’s what you do with it that counts!” Turns out that the first statement very much does matter when it comes to cell free DNA. It is becoming clearer that the size of cfDNA and ctDNA is information that […]

19 10, 2017

@Illumina Nextera Flex

By | October 19th, 2017|Categories: Exomes and amplicons, Library Prep, Methods and applications, Next-generation sequencing|1 Comment

My lab has been a long-time user of Illumina’s transposase exomes for the very simple reason that the 50ng input has been the lowest on the market for number of years*. This made it attractive for cancer samples where we are really limited on DNA availability; […]

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