I’ve been meaning to write up a post on a BioRxiv report from earlier this year: “Scaling single cell transcriptomics through split pool barcoding”1. The Seelig Lab at the University of Washington have developed a single-cell RNA sequencing method to enable labelling RNA molecules with cell-of-origin information using […]
I recently had a days training at Oxford Nanopore’s labs on their new cDNA-Seq kit and ran the resulting libraries on the GridION (one of the first customers to do so). A lot of people are watching RNA-Seq developments at ONT very closely. The London […]
This is a first for me as I’ve not finished this post at all! Instead I’m going to use this as a space to document my ideas, and experiences, in bringing Oxford Nanopore’s RNA-seq into my core lab at the CRUK Cambridge Institute. I’m hoping […]
Introducing NovaSeq This is the second part of my “everything you wanted to know about NovaSeq blog and in it I’ll finish up by discussing some of the challenges the instrument presents for Core Labs and their users, and the costs of running NovaSeq compared to […]
I’m predicting that 2017 will be the breakthrough year for nanopore sequencing. Having seen what’s been going on over the last 12-24 months I’m pretty confident that we’ll see at least one NGS niche being taken over by Nanopore sequencing in 2017. Whether this will […]
