5 05, 2017

Running @nanopore RNA-seq in a core lab

By | May 5th, 2017|Categories: Core facilities, Nanopore sequencing|Tags: |0 Comments

This is a first for me as I’ve not finished this post at all! Instead I’m going to use this as a space to document my ideas, and experiences, in bringing Oxford Nanopore’s RNA-seq into my core lab at the CRUK Cambridge Institute. I’m hoping […]

24 04, 2017

Update on @illumina index-swapping: better barcode design

By | April 24th, 2017|Categories: "Experimental design controls etc", Core facilities, Methods and applications, Next-generation sequencing|Tags: , , |2 Comments

Last week I followed up on the index-swapping issue after Illumina released their white paper and also covered what Ethan Linck at The Molecular Ecologist had posted about the Sinha et al BioRxiv paper. In that post I said I’d write a follow-up post about index design over the weekend – here it is! […]

21 04, 2017

Update on @illumina index-swapping

By | April 21st, 2017|Categories: "Experimental design controls etc", Core facilities, Design, Methods and applications, Next-generation sequencing|0 Comments

Illumina released a white paper this week describing their efforts to understand the index-swapping issues with the ExAmp chemistry. This story has been covered many times and got lots of Tweets in the days since the BioRxiv paper from Stanford was uploaded. Megan Molteni at Wired wrote a lengthy […]

28 03, 2017

What does RNA-Seq mean to you

By | March 28th, 2017|Categories: Core facilities, Methods and applications, Next-generation sequencing|0 Comments

This post is a follow on from my ABRF2017 presentation this morning. Slides are available on SlideShare. RNA-Seq is the most widely published NGS method, but what do researchers mean by the term “RNA-Seq”? Virtually none of them mean sequencing of RNA. Most are talking […]

17 03, 2017

MinION or GridION for your core lab

By | March 17th, 2017|Categories: Core facilities, Nanopore sequencing|2 Comments

Clive Brown presented a webinar on Tuesday with multiple updates on Oxford Nanopore technology, Keith Robison covered them all over at OmicsOmics, but I’ll just be focussing on the launch of GridION X5. If you want to know more about GridION, PromethION, Throughput, 1D^2 Chemistry, Basecalling, the FPGA Basecalling Accelerator, and a […]

22 02, 2017

NeoPrep is dead….what does this mean for Illumina

By | February 22nd, 2017|Categories: Core facilities, Nanopore sequencing, Next-generation sequencing|Tags: |0 Comments

Illumina have finally dropped the NeoPrep from their lineup after a short but pretty rocky road. Launched at AGBT15 the instrument promised hassle free library prep automation for users that did not require full 96well plate production-scale solutions. Early access users I spoke to at […]

26 01, 2017

(almost) everything you wanted to know about @illumina NovaSeq…and some stuff you didn’t

By | January 26th, 2017|Categories: Core facilities, Next-generation sequencing|0 Comments

Introducing NovaSeq This is the first part of a 2 part blog. Part 1 “everything you wanted to know about NovaSeq” covers the technical aspects of NovaSeq, Illumina’s latest sequencer launched on Jan 9th 2017. The instrument is very clearly the next proper step in Illumina technology, which […]

9 02, 2014

Wet-lab, dry-lab or moist-lab

By | February 9th, 2014|Categories: Core facilities|0 Comments

Wet-lab: your traditional research lab with lots going on at the bench (often collaborating with dry-labs).Dry-lab: A computational or mathematical lab focusing on analysis of other peoples data (sometimes collaborating with dry-labs).Moist-lab: the new style of lab with both wet- and dry-staff, working together with […]

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