17 05, 2018

Size matters…for cfDNA (and ctDNA)

By | May 17th, 2018|Categories: ctDNA, Library Prep, Methods and applications, Next-generation sequencing|0 Comments

“It’s not the size that matters, it’s what you do with it that counts!” Turns out that the first statement very much does matter when it comes to cell free DNA. It is becoming clearer that the size of cfDNA and ctDNA is information that […]

1 05, 2018

Biotin health supplements may affect lab tests

By | May 1st, 2018|Categories: ctDNA, Diagnostics Tech, Methods and applications, Other stuff|0 Comments

The FDA issued a safety communication today on the risk that biotin supplements “can significantly interfere with certain lab tests and cause incorrect test results”. The FDA communiqué mentions an increase in reported adverse events, and even one death, because of Biotin interference. Biotin interferes with some […]

7 02, 2018

Transplant patients may help to unlock the secrets of ctDNA

By | February 7th, 2018|Categories: ctDNA, Next-generation sequencing|0 Comments

Can transplant genomics help better understand ctDNA? Circulating cell free (cfDNA) of tumour (ctDNA) is released by apoptotic and necrotic cells into blood and other bodily fluids. It has transformed NIPT and is transforming Cancer medicine. However the biology behind it’s release is still not […]

8 11, 2017

Error-corrected ctDNA sequencing for mutation and CNV using UMIs

By | November 8th, 2017|Categories: ctDNA, Exomes and amplicons, Methods and applications, Next-generation sequencing|2 Comments

A recent BioRxiv report from the Gerlinger group at ICR describes a targeted ctDNA sequencing method that uses error correcting UMIs to achieve 100% sensitivity for mutant allele frequencies of >0.15%, and 87% at >0.075%, and reduce false-positive mutation calls by 98.6%, without adversely affecting the […]

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