10 02, 2017

Finding a service provider on Genohub…”quality guaranteed”

By | February 10th, 2017|Categories: Commissioned content, Next-generation sequencing, Other stuff|0 Comments

This article was commissioned by Genohub. If you are looking for someone to run a next-generation sequencing project for you then hopefully many of the readers of the CoreGenomics blog will have seen the googlemap of NGS instruments (watch Enseqlopedia.com/NGS-mapped for a major refresh coming soon). However the […]

10 02, 2017

Fingerprinting for multiplexed single-cell RNA-seq

By | February 10th, 2017|Categories: "Experimental design controls etc", "My almost"..., 10X Genomics, Methods and applications, Next-generation sequencing, Single-cell sequencing|Tags: |0 Comments

In this post I describe an idea for using SNP fingerprinting for single-cell RNA-Seq to identify which sample each individual cell comes from in a multiplexed library prep. The reason for my thinking about this is that single-cell experiments are expensive. Although the cost-per-cell can […]

1 02, 2017

Single-cell copy number analysis with SCI-Seq

By | February 1st, 2017|Categories: Next-generation sequencing, Single-cell sequencing|0 Comments

Andrew Adey’s group at the Department of Molecular & Medical Genetics in Oregon Health & Science University published SCI-seq in Nature Methods earlier this week (it has been on the BioRxiv since July). SCI-Seq is a method for single-cell copy number analysis that is likely […]

26 01, 2017

(almost) everything you wanted to know about @illumina NovaSeq…and some stuff you didn’t

By | January 26th, 2017|Categories: Core facilities, Next-generation sequencing|3 Comments

Introducing NovaSeq This is the first part of a 2 part blog. Part 1 “everything you wanted to know about NovaSeq” covers the technical aspects of NovaSeq, Illumina’s latest sequencer launched on Jan 9th 2017. The instrument is very clearly the next proper step in Illumina technology, which […]

5 01, 2017

My predictions for Illumina’s JP Morgan announcments

By | January 5th, 2017|Categories: Next-generation sequencing, Other stuff|4 Comments

I thought I’d follow Keith’s lead and make my own suggestions for what might be revealed during Illumina’s presentation at this years JPMorgan event. All the great announcements, by Illumina and everyone else, used to be made at the AGBT meeting in February and that gave […]

15 12, 2016

The 5 hardest things about single-cell sequencing

By | December 15th, 2016|Categories: Next-generation sequencing, Single-cell sequencing|2 Comments

Single-cell sequencing is making waves with many exciting developments over the last couple of years. Hardware is available from the companies like 10X Genomics, Fluidigm, Wafergen, BioRad (see GenomeWeb), RainDance (see GenomeWeb), Dolomite Bio. Chemistry for RNA and Genome sequencing such as SMART-Seq V4 and TruPrime, opens up all sorts […]

13 12, 2016

Oxford Nanopore – a billion dollar valuation!

By | December 13th, 2016|Categories: Next-generation sequencing|0 Comments

The latest round of fundraising by ONT brought in over $100 million, that makes a total of around $X million invested over the last X years. This round of funding was led by GT Healthcare, a Hong Kong based investment group. According to the Financial Times […]

11 12, 2016

The new sequencer from Qiagen

By | December 11th, 2016|Categories: Next-generation sequencing|1 Comment

Qiagen are now finally selling the GeneReader after a hiatus of several years. This was caused by issues getting a working instrument out of the original IBS technology, and also held, temporarily, up by recent Illumina litigation. GenomeWeb covered the relaunch back in November. Qiagens updated […]

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