The Oxford Nanopore MinION is generating some truly staggering read-lengths.
Keith Robison has summarised many of the major advances of the past few months; MinKNOW increasing yield by reducing pore blockage with a “nano-Heimlich manoeuvre” which increases yields to 10Gb (ONT’s CSO Clive Brown has self reported 20Gb), the SCRAPPIE basecaller reducing errors to 1 in 500, direct methylation analysis and of course Josh’s huge read.
Robison says “the highest output desktop sequencer is now MinION!”
Read length distribution of last week's @nanopore E. coli K-12 run. Total: 5Gb output, 63.8kb N50, longest full-length alignment 771kb. pic.twitter.com/lFkoHLrLoB
— Nick Loman (@pathogenomenick) March 6, 2017
Poll updated because of @pathogenomenick post about 882kb!
How long until someone gets the 1st 1Mb read on @nanopore MinION?
— James Hadfield (@coregenomics) March 8, 2017
So far the response is pretty bullish that 1Mb is just around the corner. I even had to update the poll because of Nicks notice that they’s got an 882kb read last night. Thats an increase of 111kb in under 48 hours!?!
@pathogenomenick @Clive_G_Brown @coregenomics @nanopore !! Soon 1 Mb will be routine….this is amazing.
— Mark Akeson (@AkesonUCSC) March 8, 2017
I think Mark Akeson’s reply sums it up perfectly!
Where is ONT headed?
If you’d like to know more bout the otput on minION (and hopefully get an update on promethION) then Clive’s presenting a webinar on MinION performance next week on March 14th 3pm GMT.
MinION performance is moving fast. Find out how, and more at @clive_g_brown webcast on 14 March 3pm GMT. Register https://t.co/9vrtBpqJm2 pic.twitter.com/bBIz72cSAY
— Oxford Nanopore (@nanopore) February 24, 2017
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