Ricki Lewis (Genetic linkage blog) wrote a great guest post for Scientific American on what exome sequencing can’t do. It seems timely considering the explosion of interest in exome sequencing and exome arrays. Not so long ago most people I knew still talked about junk […]
Ion Torrent released AmpliSeq 2.0 a little while ago. The biggest change is an increase to 3072 amplicons per pool. I saw a Life Tech slide deck which had up to 6144 amplicons pre pool so there looks like more room for improvment.Who knows when […]
We have just had an open day at our institute, we had Science Week here in the UK a month ago and I went to my son’s school to talk about why people have differently coloured eyes. I like communicating science to other people; at […]
The launch of HiSeq 2500 generated a buzz as it came hot on the heels of the Ion Proton. Both instruments will allow users to generate a genome in a day. HiSeq 2500 was launched with a 127Gb in 24 hours spec. Current specs on […]
About two years ago when we started using Fluidigm Access Array sequencing we noticed something in the reads that was a bit of a surprise, although not totally unexpected once we realised what was going on. We were amplifying all the exons in seven cancer […]
Someone recently asked me “How do SPRI beads work” and I realised I was not completely sure so I went to find out. My lab uses kits. Lots and lots of kits; kits for DNA extraction, kits for PCR, kits for NGS library prep […]
I just organised and hosted an NGS conference for 150 people in Cambridge which was well attended, had interesting talks and received positive feedback. It was a lot of work getting everything organised so I thought I’d put together my thoughts on the whole thing […]
Over the past two days I have hosted the 3rd CRI/Sanger NGS workshop at CRI. I hosted the first of these in 2009 and the demand for information about NGS technologies and applications appears to be greater today than it was back then. We had […]
Over at GenomeWeb, everyone’s favourite after CoreGenomics ;-), there is some coverage of an Illumina conference call. One statistic stood out for me and that was the “average annualised consumable utilisation of HiSeq”, I think this is how much each HiSeq owner spends on consumables. […]
My collaborator Nick Loman is lead author on a Nature Biotechnology paper released today. Performance comparison of benchtop high-throughput sequencing platforms. I have been involved in several performance comparisons in the past and the one question people ask when they read papers like this is […]
