3 10, 2017

DNA in vellum proves authenticity of paintings

By | October 3rd, 2017|Categories: Next-generation sequencing, Other stuff|0 Comments

A short article in todays Times quotes William Cowley parchment makers as saying velum provides the only foolproof way for detecting art forgery. Very simply an artist creates their work on vellum and retains a sample for genetic comparison should a suspected forgery come to […]

21 07, 2017

DAP-Seq for higher-throughput transcription factor analysis

By | July 21st, 2017|Categories: Methods and applications, Next-generation sequencing|0 Comments

The Ecker lab at the Salk Institute published a method for very high-throuphut transcription factor analysis in Nature Protocols yesterday: Mapping genome-wide transcription-factor binding sites using DAP-seq. The method is elegantly simple in its concept; PCR-free libraries from native gDNA are hybridised with affinity-tagged in vitro-expressed transcription factors, […]

12 07, 2017

ctDNA analysis from 250pg

By | July 12th, 2017|Categories: Methods and applications, Next-generation sequencing|2 Comments

A recent paper in Nature Scientific Reports from Maurice Jansen’s group at the Erasmus Medical Centre in Rotterdam presents ctDNA analysis by targeted NGS from just 250pg of input cfDNA . This is a very small amount of material, and although the authors do discuss […]

10 07, 2017

Cancer amplicon nanopore sequencing

By | July 10th, 2017|Categories: Uncategorized|0 Comments

Prof Yutaka Suzuki’s lab at the University of Tokyo published a paper last week in DNA research: Sequencing and phasing cancer mutations in lung cancers using a long-read portable sequencer. In the paper they described their work detecting SNVs in EGFR, KRAS, NRAS and NF1, and EML4-ALK, […]

22 05, 2017

#singlecell seance at the Earlham Institute

By | May 22nd, 2017|Categories: Uncategorized|0 Comments

Iain Macaulay organised a #singlecell meeting at Norwich’s Earlham Institute earlier this month. The meeting was fun and there were some great presentations during a day of talks and discussion on the development and application of single cell technologies. 100+ #singlecell analysis methods at www.tinyurl.com/mqabyur One that […]

11 05, 2017

Update on @illumina index-swapping: better libraries

By | May 11th, 2017|Categories: Methods and applications, Next-generation sequencing|Tags: |3 Comments

Index-swapping appears to be driven by excess adapter/primer (Illumina whitepaper). The take-home messages are 1) to use UDIs (unique dual-indexes), and 2) clean-up your libraries to remove left-over adapter/primer. Later in this post I’m going to work through one solution for getting rid of any remaining adapter/primer in an NGS library; but […]

Load More Posts