15 06, 2013

5hmC on Illumina 450k’s: analysis without breaking the bank?

By | June 15th, 2013|Categories: Methods and applications, Next-generation sequencing|0 Comments

Epigenetics is one of the most exciting areas of research and promises to impact all sorts of biological systems. DNA methylation has recently been shown to come in methylcytosine (mC) and 5-hydroxymethylcytosine (5hmC) flavours. Usually analysis of mC is carried out using bisulfite conversion or […]

22 05, 2013

Moleculo data presented at Illumina Scientific Summit

By | May 22nd, 2013|Categories: Methods and applications, Next-generation sequencing|0 Comments

Geoff Smith from Illumina presented a great piece of work his group have been collaborating on with Jill Banfield’s research group at the University of California Berkely, Department of Earth and Planetary sciences. Geoff included a discussion on their use of Moleculo.They have been using […]

13 04, 2013

2D RNA-seq rocks!

By | April 13th, 2013|Categories: Methods and applications, Next-generation sequencing|1 Comment

RNA-seq is getting very cool over in Sweden’s SciLifeLab. A recent press release announces that Joakim Lundeberg (Royal Institute of Technology) Jonas Frisén and Patrik StÃ¥hl (Karolinska Institutet) were awarded £1.8M from the Knut and Alice Wallenberg Foundation to study Brain biology. They have developed […]

7 04, 2013

ctExome-seq: Circulating tumour exomes as a non-invasive method to track tumour evolution during treatment

By | April 7th, 2013|Categories: Exomes and amplicons, Methods and applications, Next-generation sequencing|3 Comments

Monitoring of cancer patients is an important part of their treatment and this is traditionally done with tests like Computed Tomography imaging (CT) or biomarker analysis. NGS of cancer amplicons, exomes and/or genomes is being discussed as a realistic addition, and possibly an alternative, to […]

23 01, 2013

Mate-Pair made possible?

By | January 23rd, 2013|Categories: Methods and applications|3 Comments

Mate-Pair sequencing has been used from the early days of genome sequencing to help with final assembly. The technique creates libraries with much larger inserts than the standard fragment library prep, even 10Kb or more. However making these libraries has never been easy and the […]

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