13 04, 2013

2D RNA-seq rocks!

By | April 13th, 2013|Categories: Methods and applications, Next-generation sequencing|1 Comment

RNA-seq is getting very cool over in Sweden’s SciLifeLab. A recent press release announces that Joakim Lundeberg (Royal Institute of Technology) Jonas Frisén and Patrik StÃ¥hl (Karolinska Institutet) were awarded £1.8M from the Knut and Alice Wallenberg Foundation to study Brain biology. They have developed […]

7 04, 2013

ctExome-seq: Circulating tumour exomes as a non-invasive method to track tumour evolution during treatment

By | April 7th, 2013|Categories: Exomes and amplicons, Methods and applications, Next-generation sequencing|3 Comments

Monitoring of cancer patients is an important part of their treatment and this is traditionally done with tests like Computed Tomography imaging (CT) or biomarker analysis. NGS of cancer amplicons, exomes and/or genomes is being discussed as a realistic addition, and possibly an alternative, to […]

23 01, 2013

Mate-Pair made possible?

By | January 23rd, 2013|Categories: Methods and applications|3 Comments

Mate-Pair sequencing has been used from the early days of genome sequencing to help with final assembly. The technique creates libraries with much larger inserts than the standard fragment library prep, even 10Kb or more. However making these libraries has never been easy and the […]

28 03, 2012

What is “total RNA”?

By | March 28th, 2012|Categories: Methods and applications, Other stuff|4 Comments

I wrote this post after a recent experimental design discussion where we went round and round discussing RNA analysis.There are many different RNA molecules in a cell. When I was studying there were only three, mRNA, ribosomal RNA and tRNA. Simple! However over the last […]

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