Update 25th April: Illumina have released a support bulletin “Best Practices for High Sensitivity Applications: Minimizing Sample Carryover”, this is accessible through Illumina’s website, or SEQanswers. You’ll need an Illumina login!Illumina say that sample carryover is more likely to have an effect on very low […]
RNA-seq is getting very cool over in Sweden’s SciLifeLab. A recent press release announces that Joakim Lundeberg (Royal Institute of Technology) Jonas Frisén and Patrik StÃ¥hl (Karolinska Institutet) were awarded £1.8M from the Knut and Alice Wallenberg Foundation to study Brain biology. They have developed […]
One of the great things about ENCODE was the amount of effort put into standardising how different groups did their experiments. As any core manager knows a good SOP is a huge step to high-quality data. The ENCODE consortium put together guidelines for ChIP, RNA […]
Monitoring of cancer patients is an important part of their treatment and this is traditionally done with tests like Computed Tomography imaging (CT) or biomarker analysis. NGS of cancer amplicons, exomes and/or genomes is being discussed as a realistic addition, and possibly an alternative, to […]
Sequencing from small amounts of nucleic acid is opening up new areas of research. Whilst microarray analysis of single cells has been performed in the past analysing the genome from such a small amount of DNA has been an almost impossible task. Equally, analysis of […]
We have some old lab equipment we are getting rid of much of it will be recycled into other University departments. Some will be sold to equipment resellers like Biagen, Alliance Analytical, and Harlow Scientific. Some of it may even end up in the skip. […]
Today I was given an exclusive preview from the newest nanopore sequencing company on the planet, Norfolk Nanopore Technology. The new “Polonopore”technology has been incubating in the Norwich Research Park BioIncubator under the same roof as TGAC. NNT’s brand spanking new nanopore sequencer could mean […]
Updated after reading this post on GenomeWeb!Oxford University and the NHS announced the UK’s first next-generation sequencing based test for Cancer. The test uses Life Technologies Ion Torrent PGM and AmpliSeq to assay 46 gene “hotspots”, but should increase to around 150 genes in the […]
A colleague and I were discussing the woes of post-docs trying to find their next job, or a tenure-track position. My colleague was relating the story of a mutual friend who recently published an excellent first-tier publication and was still having problems finding a junior […]
I would like to move from microarray based genotyping to NGS for copy-number and loss-of-heterozygosity analysis. The copy-number bit should be relatively simple and I hope we’ll have done this by the end of the year, however the LOH analysis is more complex as it […]
