In yesterdays Fast Company piece Christina Farr (on Twitter) gives a very nice write up of Illumina’s history and where they are going with respect to bringing DNA sequencing into the clinic. I really liked the piece and wanted to share my thoughts after reading […]
The Genomics community is pushing ahead fast on single-cell analysis methods as these are revolutionising how we approach biological questions. Unfortunately my registration went in too late for the meeting running at the Sanger Institute this week (Follow #SCG16 on Twitter), but the Fluidigm pre-meeting was a […]
Anyone that’s been reading Core-Genomics will have seen my interest in the technology from 10X Genomics. I’ve been watching and waiting for publications to come out to get a better understanding of how people are using the technology and thought you might like my current […]
This post follows on from my previous one explaining the 10X Genomics single-cell mRNA-seq assay. This time round I’m really reviewing the method as described in a paper recently put up on the BioRxiv by 10X’s Deanna Church and David Jaffe: Direct determination of diploid genome […]
The amount of power used in a genome analysis is not something I’d ever thought of until I heard about Optalysys, a company developing optical computing that has the potential to be 90% more energy-efficient and 20X faster than than standard (electronic) compute infrastructure. Read on if you are […]
It is almost time for the kick off at Genome Science, probably the best organised academic conference in the UK. It runs from August 30th to September 1st next week and sadly I can’t be there (just returned from holidays and too much going on). You can hear from […]
10X Genomics have been very successful in developing their gel-bead droplet technology for phased genome sequencing and more recently, single-cell 3’mRNA-seq. I’ve posted about their technology before (at AGBT2016, and March and November 2015) and based most of what I’ve written on discussion with 10X […]
This article was commissioned by Illumina Inc.The most common NGS method we discuss in our weekly experimental design meeting is RNA-seq. Nearly all projects will use it at some point to delve deeply into hypothesis driven questions, or simply as a tool to go fishing for new […]
A new genome amplification technology from Expedeon/Sygnis: TruePrime looks like it might work great for single-cell and low-input anlyses – particularly copy number. TruePrimer is a primer-free multiple displacement amplification technology. It uses the well established phi29 DNA polymerase and a new TthPrimPol primase, which […]
A recent paper from Mike Quail’s group at the Sanger Institute compares 9 different library prep kits for WGS. In Quantitation of next generation sequencing library preparation protocol efficiencies using droplet digital PCR assays – a systematic comparison of DNA library preparation kits for Illumina […]
