Researchers in the group of Yolanda Sanz in Spain deposited a preliminary MinION study describing the bioRxiv of 16S rDNA amplicon sequencing from a mock microbial community composed of genomic DNA from 20 different bacterial species (BEI Resources).Experimental workflow: 1.5kb amplicons were generated from 16S […]
Updated after a conversation with Illumina: it turns out pricing for V4 was released early in an email from Christian Henry (Illumina SVP & COO) on Feb 25th. Single end pricing is now compared below and list pricing for all reagents has been used in […]
I had a fun time at Jason Carroll’s group retreat in sunny Cromer a few weeks ago. I was invited to present some work we’d been doing on using Thruplex for ChIP-seq library prep and there was a lot of great science being discussed. One of […]
A recent PLoS One paper is perhaps the latest battle in the Illumina vs LifeTech war. The latest paper in PLOS ONE presents a comparison of MiSeq and Proton PI sequencing to detect chromosome abnormalities in self-aborted foetuses: Chen et al: Performance Comparison between Rapid […]
Joakim Lundeberg at the Science for Life Laboratory, Stockholm, Sweden has published a nice paper in PLoS One on the impact of RNA degradation on the quality of RNA-seq and differential expression results: Sigurgeirsson, Emanuelsson & Lundeberg: Sequencing Degraded RNA Addressed by 3′ Tag Counting. […]
We’ve been running Illumina NGS in my lab for six and a half years and have almost 8000 libraries in our -20 going back almost to the start, to number 300 in fact. Occasionally we have users requesting us to rerun these old libraries and […]
I’m going to start this post by saying something that may be controversial “splicing analysis is a niche, strandedness is useful in a minority of cases and that most RNA-seq users are only interested in getting Affy-like 3′ biased differential gene expression data”.I’m constantly looking […]
An awesome demonstration of what’s possible when people think outside the box: Highly Multiplexed Subcellular RNA Sequencing in Situ in Science this week demonstrates in situ RNA-seq of single cells. FISSEQ (fluorescent in situ RNA sequencing) was developed in the lab of George Church who’s […]
The most recent ctDNA paper verifies its utility as a “broadly applicable, sensitive, and specific biomarker†across “multiple different types of cancer†and useful in cancer research and medicine: Detection of Circulating Tumor DNA in Early- and Late-Stage Human Malignancies
 by Bettegowda et al Sci […]
Jacques Retief (Sr. Manager Scientific Affairs at Illumina) has produced a phenomenal poster with graphics for over NGS 50 methods. If you’ve ever wanted to know how MAINE-seq, smMIP, ATAC-seq or just about anything else worked then Jaques helpfully provides a graphic of each method […]
