The launch of HiSeq 2500 generated a buzz as it came hot on the heels of the Ion Proton. Both instruments will allow users to generate a genome in a day. HiSeq 2500 was launched with a 127Gb in 24 hours spec. Current specs on […]
About two years ago when we started using Fluidigm Access Array sequencing we noticed something in the reads that was a bit of a surprise, although not totally unexpected once we realised what was going on. We were amplifying all the exons in seven cancer […]
Over the past two days I have hosted the 3rd CRI/Sanger NGS workshop at CRI. I hosted the first of these in 2009 and the demand for information about NGS technologies and applications appears to be greater today than it was back then. We had […]
Over at GenomeWeb, everyone’s favourite after CoreGenomics ;-), there is some coverage of an Illumina conference call. One statistic stood out for me and that was the “average annualised consumable utilisation of HiSeq”, I think this is how much each HiSeq owner spends on consumables. […]
My collaborator Nick Loman is lead author on a Nature Biotechnology paper released today. Performance comparison of benchtop high-throughput sequencing platforms. I have been involved in several performance comparisons in the past and the one question people ask when they read papers like this is […]
I used to ride a BMWR1200RT on my daily commute from Wymondham to Cambridge. What I really wanted was an R1200GS like Ewan and Charlie rode around the world on during their easterly 19,000-mile London to New York trip through Europe and Asia to Alaska […]
I have previously blogged about amplicon sequencing (here, here and here) because I think it is one of the most exciting developments for clinical application of next-generation sequencing. Anyone can access the technology but there is a lot of choice about which system to use […]
Illumina and Affymetrix both recently released Exome microarrays. The “death” of Gene expression microarrays: This is an interesting development considering there has been a lot of debate over the “death of arrays†in the last year or so. It does looks like 3’ expression arrays […]
One of the highlights at AGBT for me was Illumina’s announcement of a 687bp “perfect” read at AGBT on MiSeq. This was the longest read in a PE400bp dataset, the read had a 113bp overlap sequence where both ends gave the same basecalls. My first […]
AGBT is over and what a meeting it was. I have never been to a conference where it felt like everyone, and it really did feel like everyone was talking about the same thing over lunch. Oxford Nanopore made a big impact.There has been some […]
