A recent Lancet paper (Whole-genome sequencing for analysis of an outbreak of meticillin-resistant Staphylococcusaureus: a descriptive study) describes work in the Rosie’s special care bay unit at Addenbrookes hospital in Cambridge (just across the road from where I work).The group studied a putative outbreak of […]
I am often asked for a good NGS reference, or references that explain the technology used in next generation sequencing experiments. I have gathered together a list of my favourites over the past few years and thought readers of this blog might like to see […]
Prof Steve Jackson presented a great seminar today at CRI on his labs work on DNA damage repair. One of the numbers that jumped out at me was 1,000,000, that is 1M DNA damage events per cell per day.Just think, even with all that going […]
Many many years ago I started up an Affymetrix microarray core lab. One of the early presentations on the 10k genotyping arrays was an epiphany for me. It was clear from the data that copy number would be easily discernible from the technology. I also […]
Tycho Brae was recently exhumed for the second time. The astronomer died suddenly in 1601 and analysis of hair from a 1901 exhumation suggested he may have been poisoned. The recent exhumation by the University of Southern Denmark disproved this theory, however what exactly did […]
Our MiSeq has just been replaced after some performance issues and we are now getting ready to start running the upgraded version with the newest chemistry. Hopefully we’ll be getting the 10Gb+ that Illumina reported some users are achieving.In a GenomeWeb article yesterday Illumina responded […]
Many groups are working on next-generation sequencing tests for mutation profiling of tumours. There are two main approaches, amplicon sequencing (reviewed in a previous post) and in-solution capture capture (reviewed here and here); both of which only look at DNA which is not immediately limiting, […]
Go back to the summary of the CRI symposium.Carlos Caldas, Functional genomics of cancer: perturbation experiments in the lab and in the clinic. Carlos is a senior group leader at the Cancer Research UK Cambridge Research Institute where I work, he is also a practising […]
Go back to the summary of the CRI symposium.Barbara Wold, The genomics discovery path from TCGA and TARGET to the clinic…and back. Unfortunately Dr Wold flew all the way over, escaping hurricane Sandy but was kept in her hotel room by flu.Keith Peters, was Regius […]
Go back to the summary of the CRI symposium.Overall summary: Is it possible to move the goal-posts and say that a clinical “test†no longer revolves around the actual sequencing of a cancer gene, as in the case of BRCA1/2 screening, but is now more […]
