Update from GenomeWeb at the bottom!Get ready for millions of minions! ONT have announced an early-access program for the MinIon (GridION later) and it is almost free to access. I’m sure they will see huge demand from eager NGS users. But who will have projects […]
A few years ago one of our junior group leaders made an interesting appointment; he recruited a bioinformatician into a research assistant role. Every lab has someone, or several people, who keep the lab running. They are the people making sure cells get cultured, supporting […]
Genomics England is steaming ahead to sequence 100,000 genomes from NHS patients. Today Genomics England and Illumina announced their intention to start the 1st 10,000 genomes as part of a seqeucning contract run by Illumina. Set up by the Department of Health and announced […]
Here’s a bold statement: “I believe almost all NGS experiments would be better off run as a single pool of samples across multiple lanes on a sequencer.” So why do many users still run single-sample per-lane experiments or stick to multiplexes that give them a defined […]
In a post earlier this week I talked about the hacking of a MiSeq run by MadsAlbertsen, one comment on the post drew my attention to another paper I’d missed where the authors hacked their MiSeq to perform 600bp reads (PE300). Considering this was a […]
Illumina acquired Verinata earlier this year and their Verif prenatal test is a non-invasive one that detects foetal aneuploidies as early as 10 weeks. Many others are developing or selling similar tests and the real excitement for me (as I already have kids and don’t […]
We’ve been asked a question many times over the years “should you count paired-end sequencing as one read or two?†“Who cares†I hear you cry. But if you ask someone to give you back 200M reads for a sample and they give you 100M […]
I’ll start off by saying not quite, but you can read on to get an idea on how to increase read length of a MiSeq 500cycle v2 kit to get 600bp of data. MadsAlbertsen posted on a SEQanswers thread about their protocol to squeeze a […]
Update: …see the bottom of the post for more coverage on this “sting”GenomeWeb has coverage of a story all of us should take a look at. A fake manuscript produced by a journalist from Science was accepted by 157 open-access publishers, a damning indictment of […]
Analysis of circulating DNA has had a major impact in the last couple of years with 100s of publications in the last few years. I’ve previously posted about work at the Institute on circulating tumour DNA analysis of amplicons and exomes, but what about RNA?RNAs […]
