DMSO is used in many protocols as a ‘universal solvent’, it’s great for solublising small molecules and you’ve almost certainly used DMSO when freezing cells or in PCR. Unfortunately it’s relative ubiquity of use in research labs means it’s negative impacts on some compounds can […]
The Circos plot is one of my favourite visualisations of genomic data, it’s easy to be blown away by the images our microscopy core produce and genomic data is usually quite a let down by comparison. But the Circos plot is not the first visualisation […]
Wow there is a lot of choice when it comes to buying something as simple as an NGS library prep kit. With Illumain in mind (as always) I found at least 17 providers. includding: Affymetrix, Agilent, Beckman, Bioo, Enzymatics, Epigentek, Illumina, Kapa, Lucigen, Mclab, Milipore, NEB, […]
It’s holiday season every season with pretty much every academic pursuit. We’re very lucky being ale to escape to some wonderful places to discuss the work we do and face-to-face really is the best way. Sometimes choosing which conference to go to can be a […]
I’m continuing a series of posts that will highlight small advances we’ve made in the lab that are unlikely to be published in their own right, this time its the turn of SPRI-bead clean-ups and how we used them to rescue some samples that would […]
Updated after a conversation with Illumina: it turns out pricing for V4 was released early in an email from Christian Henry (Illumina SVP & COO) on Feb 25th. Single end pricing is now compared below and list pricing for all reagents has been used in […]
Cancer Research UK science has been turned into a game for your mobile: Genes in Space. The aim is to collect space rocks; to do this you need to map a route through the densest regions in space then fly though and catch as many […]
I had a fun time at Jason Carroll’s group retreat in sunny Cromer a few weeks ago. I was invited to present some work we’d been doing on using Thruplex for ChIP-seq library prep and there was a lot of great science being discussed. One of […]
Illumina are probably going to announce their latest sequencer today and Core Genomics was heard about it before anyone else…MiSeq X. Similar in name to the HiSeq X Ten the MiSeq X borrows many of the improvements seen in it’s big brother including new optics, […]
A recent PLoS One paper is perhaps the latest battle in the Illumina vs LifeTech war. The latest paper in PLOS ONE presents a comparison of MiSeq and Proton PI sequencing to detect chromosome abnormalities in self-aborted foetuses: Chen et al: Performance Comparison between Rapid […]
