I’ve been finding out more about exomes: specifically QC analysis using HS Metrics in Picard. There’s loads of useful metrics and I’m hoping to get to a point that I can explain these to users here and also look at the results to try and […]
Why does blogger’s spell check try to correct ‘blog’, and why does it suggest ‘bog’ or ‘blag’. Are they telling me I’m writing s**t or trying to get something for free?Please fix this one blogger.com.
This morning someone made the 500,000th page view on the CoreGenomics blog. It amazes me that so many people are reading this and the last couple of years writing have been really good fun. I’ve met many readers and some fellow bloggers, and received lots […]
I’m speaking at the BaseSpace Apps developers conference in Bangalore tomorrow. It’s my first App and my first time in India, so I’m pretty excited about the whole thing.Earlier this year Matt Eldridge (Head of Bioinformatics) and I published a little paper on our sequencing […]
Inivata, is a new company spun out of Nitzan Rosenfelds research group at the CRUK Cambridge Institute (where I work). His group developed and published the TAm-seq method for circulating tumour DNA amplicon sequencing. The spin-out aims to develop blood tests measuring circulating tumour DNA […]
I’m continuing my exome posts with a quick observation. There have been several talks recently that I’ve seen where people present genome and exome data and highlight the drop-out of genomic regions primarily due to PCR amplification and hybridisation artefacts. They make a compelling case […]
I’ve heard lots of presentations about novel sequencing technologies, many have never arrived, some have come and gone, all have been pretty neat ideas; but so far not one has arrived that outperforms the Illumina systems many readers of this blog are using.Base4’s pyrophosphorolysis sequencing […]
Illumina recently released a new version of HCS v2.2.38 for the HiSeq. The update improves cluster definition significantly and enables low-diversity sequencing. It’s a great update and one that’s making a big impact on a couple of projects here. Better RRBS: the current RRBS protocols […]
Illumina recently released a new version of HCS v2.2.38 for the HiSeq. The update improves cluster definition significantly and enables low-diversity sequencing. It’s a great update and one that’s making a big impact on a couple of projects here. Better RRBS: the current RRBS protocols […]
I’ve been working with microarrays since 2000 and ever since RNA-seq came on the scene the writing has been on the wall. RNA-seq has so many advantages over arrays that we’ve been recommending them as the best way to generate differential gene expression data for […]
