The NGS community has been talking about the $1000 genome as a holy grail (expect it in the second half of 2014), but on the way to this the exome is looking like it will become a cornerstone of modern medical research mainly because of […]
RNA-seq is getting very cool over in Sweden’s SciLifeLab. A recent press release announces that Joakim Lundeberg (Royal Institute of Technology) Jonas Frisén and Patrik StÃ¥hl (Karolinska Institutet) were awarded £1.8M from the Knut and Alice Wallenberg Foundation to study Brain biology. They have developed […]
Monitoring of cancer patients is an important part of their treatment and this is traditionally done with tests like Computed Tomography imaging (CT) or biomarker analysis. NGS of cancer amplicons, exomes and/or genomes is being discussed as a realistic addition, and possibly an alternative, to […]
Sequencing from small amounts of nucleic acid is opening up new areas of research. Whilst microarray analysis of single cells has been performed in the past analysing the genome from such a small amount of DNA has been an almost impossible task. Equally, analysis of […]
There is lots of discussion about the $1000 genome, what it means, what its impact might be, etc.First off though deciding what a $1000 genome means seems to be almost impossible given everybody uses different ways to calculate costs. In a soon to be published […]
Illumina’s is the most widely used next-generation sequencing technology, but like all technologies it is not perfect. You’ll have to wait for their Nanopore sequencer for perfection! One challenge we have to deal with ever more with Illumina sequencing is the balance and number of […]
De novo assembly of complex genomes is still harder than many would like.For Cancer genomes de novo assembly could be the ultimate method for discovery of all somatic events. But the analysis requires long-reads or long-insert reads and this generally eats up lots of DNA […]
I like encouraging people to try to come up with new ideas. One day I want to add ‘inventor’ to my CV and get a patent, I posted a while ago about an idea that came very close but was pipped to the post by […]
There has been lots of recent activity around using NGS gene resequencing in the clinic. Although clinical DNA sequencing has been an important tool for several decades the explosion in NGS methods for amplicon resequencing has made it feasible for just about any lab to […]
One of the things I most like about my job is being allowed to think. NGS has provided a very fertile ground for innovation and lots of novel ideas have become products we use regularly today. It is sometimes to easy to forget that any […]
