Today I thought I’d give my first impressions on what HiSeq X Ten might mean, the dust has certainly not settled and yesterday there was lots of buzz about the new instruments from Illumina (see the bottom of this post for a round up of […]
JF did not disappoint at JPM: Jay Flatley announced both the HiSeq X Ten and NextSeq 500, two more instruments to add to HiSeq and MiSeq. HiSeq X Ten comes as ten “ultra-high throughput†sequencing instruments that together can generate 18Tb in three days, with […]
Here’s a bold statement: “I believe almost all NGS experiments would be better off run as a single pool of samples across multiple lanes on a sequencer.” So why do many users still run single-sample per-lane experiments or stick to multiplexes that give them a defined […]
In a post earlier this week I talked about the hacking of a MiSeq run by MadsAlbertsen, one comment on the post drew my attention to another paper I’d missed where the authors hacked their MiSeq to perform 600bp reads (PE300). Considering this was a […]
Illumina acquired Verinata earlier this year and their Verif prenatal test is a non-invasive one that detects foetal aneuploidies as early as 10 weeks. Many others are developing or selling similar tests and the real excitement for me (as I already have kids and don’t […]
We’ve been asked a question many times over the years “should you count paired-end sequencing as one read or two?†“Who cares†I hear you cry. But if you ask someone to give you back 200M reads for a sample and they give you 100M […]
I’ll start off by saying not quite, but you can read on to get an idea on how to increase read length of a MiSeq 500cycle v2 kit to get 600bp of data. MadsAlbertsen posted on a SEQanswers thread about their protocol to squeeze a […]
There was a real buzz in the community when Illumina resurrected the idea of using patterned flowcells for SBS sequencing (Keith Robison covered lots of genomics news back in January, including patterned flowcells ). One of the big problems with clustering is the need to […]
I’m often asked which sample prep method a user should consider for their experiments. In my lab we use a lot of Illumina TruSeq kits; we’ve tried other methods, and do use Rubicon’s Thruplex, but Ilumina’s end-to-end support is useful in a medium sized […]
Illumina and Life Technologies are both launching targeted RNA-seq applications which are likely to become standard tools for many labs; if the price is right. The ability to target a portion of the genome has revolutionised next-generation sequencing experiments. The analysis of exomes has exploded, […]
