One of my favourite columns in any scientific journal is Nature Methods “Points of View†by Bang Wong. The column is focused on visualisation and presentation of scientific data and I’d highly recommend it if you haven’t already seen it. Here is a link to […]
The number of samples being run in sequencing projects seems is rapidly increasing. As groups move to running replicates (why on earth we didn’t do this from day one is a little mind boggling). Most experiments today are using some form of multiplexing, commonly by […]
Illumina have released new Nextera Exome and Nextera Custom enrichment kits. These combine the rapid and simple sample prep of Nextera to in-solution genome capture and provide a straight-forward two-and-a-half day protocol. Add one day of sequencing on 2500 and you can expect your results […]
An amazing story was featured in the New York Times, the article follows Dr. Lukas Wartman from Wash U who is a leukaemia researcher who developed leukaemia himself.The Genome Institute at Wash U made a concerted effort to find out what was behind Dr Wartman’s […]
A couple of months ago Fluidigm disclosed the latest addition to their microfluidic chip technology, the C1 single-cell analysis system. At the disclosure in May the details of the C1 were a little sketchy but the system was planned to take cell suspensions, separate and […]
A couple of recent papers have demonstrated the ability to distinguish between 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) using modified bisulfite sequencing protocols. These methods are likely to make a real impact on epigenomics when combined with Bis-seq. By sequencing two genomes, Bis-seq for mC and […]
This update was added on 11 July 2012: An article on GenomeWeb made interesting reading as it talked about the current litigation between Columbia University and Illumina. In it Thomas Theuringer (Qiagen PR Director) said “We support Columbia’s litigation against Illumina, and are also entitled […]
Small RNA biases have been very well interrogated in a series of papers released in the last 12 months. The RNA ligation has been shown to be the major source of bias and the articles discussed in this post offer some simple fixes to current […]
One of the things I wanted to do with this blog was share things that make my job easier. One of the jobs I often have to do is communicate numbers quickly and effectively and a box plot can really help. I also have the […]
I’d like to highlight some research that I have been involved in, which has just been published in Science Translational Medicine; Noninvasive identification and monitoring of cancer mutations by targeted deep sequencing of plasma DNA. The work makes extensive use of Fluidigm’s Access Array and […]
