I did not arrive at London Calling until late on Thursday afternoon but what an excellent start I was given: Clive Brown’s update – where ONT are today, and what’s coming tomorrow! The Thursday afternoon, evening and Friday sessions created a palpable buzz in the […]
I’ll finish my write up of the ONT London Calling event after the bank-holiday weekend but I wanted to get this post out as I think we came up with a killer app for MinION, or even SmidgION on your iPhone: direct RNA-seq from bacterial […]
Next-generation sequencing is fantastic technology and its use has revolutionised our understanding of biology, but it is not perfect, multiple issues occur in every lab from sample extraction through to the actual sequencing. Not all of these are well enough understood to be safely ignored […]
NGS is 10…according to the latest Nature Reviews Genetics: Coming of age: ten years of next-generation sequencing technologies. Just by chance I was asked to give a talk to explain how Illumina sequencing works in a technology seminar series being delivered by the Core Heads […]
Foundation Medicine were yestoday granted US Patent 9,340,830 “Optimization of Multigene Analysis of Tumor Samples”. This is likely to stir up the can of worms that is tumour testing by NGS and is another patent in a complex landscape. The claims basically cover WGS library […]
Illumina’s X Ten was a major announcement. It arguably delivered the “$1000 genome”, kickstarted national population genomics as a science, and delivered the final blow to Complete Genomics. It is debatable as to whether Illumina needed to release X Ten at $1000, or with such […]
I’ve been a big fan of Illumina’s BaseSpace since it was launched in late 2011. It was the first truly simple to access and free to use cloud-based analysis infrastructure for NGS data. I’ve used it a lot – primarily for run monitoring, but also […]
Last year I posted about the Lander-Waterman equation used to calculate the number of reads needed to sequence a sample. I explained that this general equation (C = LN/G) can be rearranged to allow you to compute the number of reads (N) to sequence a […]
SPRI beads, generally in the form of AMPureXP beads, are almost ubiquitous in genomics applications such as library prep for NGS. The most popular thing I’ve ever written was a post on this blog four years ago: “How do SPRI beads work?” with almost 100,000 […]
I met two dads last night, one a junior doctor picking his daughter up from the same Karate class my son; the other, a consultant, at the pub after a long day (covering for junior doctors). Both of them spoke about the junior doctors strike […]
