Illumina are currently offering a demo kit for custom TruSeq capture. I thought I would try it out on some genes from COSMIC and see how easy it was to design a capture set using their DesignStudio tool. There is also a pricing calculator I […]
In the last few days there has been a mini splurge in papers reviewing capture technologies. I thought it would be useful to write an overview of these. I have been involved in several comparison papers and so am aware of the limitations of comparison […]
23andMe just launched their new programme to perform exome sequencing for individuals. The Exome80 “Personal exome sequencing” will cost just $999! This will now put pressure on some of those who have had their genomes scanned on arrays to pay up for the exome or […]
There is an enormous demand for resequencing of specific cancer loci. BRCA1 & 2, TP53, PTEN, KRAS, are already being Sanger sequenced in many labs but this process is not scalable to all cancer patients’ tumours. Some NHS hospitals do test cancer patients tumours for […]
This is the big question for many labs today, especially core facilities investing in technology for the longer term. Everyone wants to know when we might replace microarrays with NGS for applications like, differential gene expression, splicing analysis, allele specific expression, copy number variation, loss […]
The list at the bottom is occasionally edited to include new methods.There is an ever increasing number of next generation sequencing acronyms (see the link to a table at the bottom of this post), other than the commonly used ChIP-Seq and RNA-Seq most have been […]
Bang went the starter gun in August 2010 when LifeTechnologies announced the purchase of Ion Torrent for $375M (plus another $350M if certain milestones are reached).Yesterday in Nature Life Technologies and Ion Torrent published the genome of Gordon Moore of “Moore’s law†fame, plus 3 […]
The ENCODE consortium released its first set of data-standards guidelines and interestingly they are for RNA-Seq. ChIP-Seq guidelines will follow later which is a little surprising considering almost all the ENCODE data so far is ChIP-Seq (see below). In some ways I’d have preferred to […]
How a little bug got involved in a big battle: MiSeq vs IonThere has been a lot said about the recent sequencing of E coli from the recent outbreak in Germany. Over five isolates have been sequenced on almost every sequencing platform, HiSeq, 454, Ion […]
So here it is, I was given one of these at AGBT and there is a picture in the MiSeq brochure. I thought I’d share an image with anyone wondering what they look like.You can see it is very different from a HiSeq flowcell in […]
