Mate-Pair sequencing has been used from the early days of genome sequencing to help with final assembly. The technique creates libraries with much larger inserts than the standard fragment library prep, even 10Kb or more. However making these libraries has never been easy and the […]
Someone recently asked me “How do SPRI beads work” and I realised I was not completely sure so I went to find out. My lab uses kits. Lots and lots of kits; kits for DNA extraction, kits for PCR, kits for NGS library prep […]
I had a very interesting discussion over coffee yesterday at TRON, a new Translational Oncology institute in Mainz, Germany. TRON is specialising in cancer immunology and genomics, or Immunomics. One of the things they are aiming to do is develop personalised cancer vaccines by finding […]
I wrote this post after a recent experimental design discussion where we went round and round discussing RNA analysis.There are many different RNA molecules in a cell. When I was studying there were only three, mRNA, ribosomal RNA and tRNA. Simple! However over the last […]
