Sequencing from small amounts of nucleic acid is opening up new areas of research. Whilst microarray analysis of single cells has been performed in the past analysing the genome from such a small amount of DNA has been an almost impossible task. Equally, analysis of […]
There has been lots of recent activity around using NGS gene resequencing in the clinic. Although clinical DNA sequencing has been an important tool for several decades the explosion in NGS methods for amplicon resequencing has made it feasible for just about any lab to […]
Ricki Lewis (Genetic linkage blog) wrote a great guest post for Scientific American on what exome sequencing can’t do. It seems timely considering the explosion of interest in exome sequencing and exome arrays. Not so long ago most people I knew still talked about junk […]
Ion Torrent released AmpliSeq 2.0 a little while ago. The biggest change is an increase to 3072 amplicons per pool. I saw a Life Tech slide deck which had up to 6144 amplicons pre pool so there looks like more room for improvment.Who knows when […]
I have previously blogged about amplicon sequencing (here, here and here) because I think it is one of the most exciting developments for clinical application of next-generation sequencing. Anyone can access the technology but there is a lot of choice about which system to use […]
Illumina and Affymetrix both recently released Exome microarrays. The “death” of Gene expression microarrays: This is an interesting development considering there has been a lot of debate over the “death of arrays†in the last year or so. It does looks like 3’ expression arrays […]
