Cellular Research released their latest development: Resolve for single cell mRNA-seq sample-prep at under £1 per cell. A paper in today’s Science describes the method: Combinatorial labeling of single cells for gene expression cytometry. Using CytoSeq (why not Cyto-seq) 10,000 or 100,000 cells can be […]

Updated: The cost per M reads and cost per Gb figures in the original posting were wrong – damned Excel operator error! I’ve fixed them, again. Thanks to Shawn for his comments.I’ve been asked about the difference between the Illumina sequencer line-up so many times […]

The amount of RNA used in an RNA-seq library prep is often listed as a competitive advantage by kit manufacturers. As late as 2004 I used up to 30ug of RNA in a microarray prep, and even a couple of years ago 100ng was considered […]

“We live in an interesting age” or so the quote goes. It is very interesting that BGI are going to start selling sequencing technology. The coverage of Jun Wang’s (BGI CEO) JP Morgan presentation by GenomeWeb lays out what they plan to do to start […]

I was as surprised as everyone else with the most recent announcements by Illumina (JP Morgan post), especially their rapidity given that V4 chemistry is just 6months old; and I quickly sketched out my initial thoughts at the beginning of the week. There’s been lots […]

For those that are interested you can listen to the presentation by Jay Flatley at JP Morgan. And if you can’t be bothered, then here are my notes (be warned they may contain inaccuracies):The last 12 months: 2014 was massive for Illumina – X Ten […]

Illumina continue to push hard on making DNA sequencing cheaper and easier to access for everyone. The announcements at last night’s JP Morgan included a smaller X Ten, HiSeq 3000/4000 and NextSeq 550. I’m not going into much detail about the instruments, you can get […]

There is a whole big noisy discussion going on around the regulation of NGS tests, which has been covered in some detail by GenomeWeb and other bloggers. My lab does not develop tests, but I have a real interest in seeing how NGS is being […]

Shortly after writing my post on Authorship and acknowledgement of core facility work I was pointed to the GATC Top publications of 2014. Here GATC list the papers where their sequencing (and other) services have been used and acknowledged.Congratulations GATC: four Nature and one Cancer […]