I realised I’d not covered the recent droplet sequencing papers (the post was sitting in my draft blogs pile) and wanted to make sure I did after posting on single-cell RNA-seq last week. The use of droplets has some benefits over microfluidics where the current […]
A team from Columbia University present a microfluidic device that can capture single-cell lysates in picotire plates and produce single-cell 3′ tag RNA-Seq library prep at $0.10 cents per cell: Scalable microfluidics for single cell RNA printing and sequencing. They discuss two methods – RNA […]
When George Church talks about something it often turns out to be a good idea to listen. He’s been talking about in situ sequencing for many years and the technology looks to be ready for take off. It could be a niche method used by […]
A year ago I surveyed the costs of Illumina library prep and found over 15 providers; they offer generally the standard Illumina library prep method but with prices ranging from £15-£60 per sample. There was some real innovation amongst some of these kits too, enabling […]
I’m often trying to find an image for a post and it can be tough trying to find something that can be used freely. The U.S. DOE has an image gallery from the Genomic Science program, which includes archived images from the Human Genome Project. […]
Michael Snyder has a great paper published in AJRCCM: Exome Sequencing of Neonatal Blood Spots Identifies Genes Implicated in Bronchopulmonary Dysplasia (BPD). BPD is a lung disease of premature babies which appears to have a strong genetic component and was investigated using exome sequencing. The […]
Next-Generation DNA Sequencing Informatics (2nd edition), edited by Stuart M. Brown (blogger) is a book to get you well and truly started in NGS Bioinformatics. The twelve chapters cover QC, sequence alignment, assembly, transcriptome and ChIP-seq analysis, visualisation, and much else besides. For this review […]
6310 MinIONs fit inside one PacBIO!London Calling: No-one who is interested in NGS can have missed the developments from Oxford Nanopore, Twitter was on fire last week during the London Calling event and there are some great reviews of what went on for those of […]
The GIAB consortium (@GenomeInABottle) took a major step forward today when it released the first NIST reference material for Human genome sequencing, the story even made it into the New York Times. It comes at an important time when we’re moving into an era where […]
Oops, I forgot to publish this post after the meeting!So many posters at AGBT and so little time. I did not get round everything and these highlights were some of the many that caught my eye. I’d like to hope the organisers make the posters […]
