Fluidgm hosted a webinar this afternoon to describe the performance of the redesigned IFCs for medium-sized cells (10-17um), they’ve also released a whitepaper on their website describing their work. The redesigned medium-cell 96 IFCs have a >4-fold reduction in doublet rate at around 7%.This is […]
I’ve just started using Twitter polls and kicked things off with a couple asking followers whether they thought Cancer research (or diagnostics) is best investigated using whole genome sequencing, higher depth exomes, much higher depth amplicomes, or even long-reads. Please do take the poll and […]
We recently bought two HiSeq 4000 instruments to run larger RNA-seq and exome projects on. Over the next few months we’ll be transitioning other library types where we can, and think perhaps 65-85% of the work we do can be ported over from the 2500. […]
The landscape for gene expression analysis (and many other analyses) is moving away from measurements made in bulk tissues to single-cell methods. Bulk measurements are an average of the cells in the sample and so cannot truly reveal the subtleties of the biology in the […]
I thought I’d post a brief update on a story from last year where I was alerted by @MattiasAine via one of my TwitterBots about a group ripping off his Karlsson 2014 paper. The evidence was pretty damning – almost identical data, analysis, and figures […]
I’ve just posted an advert for a Principal Scientific Associate to join the Genomics Core as the Deputy Manager. This is going to be a key position in the team as we’re building building our single-cell genomics capabilities. We’re looking for someone with a PhD […]
Eric Minikel is lead author on a paper that came out in STM in January: Quantifying prion disease penetrance using large population control cohorts. The story is an incredibly personal one involving his wife Sonia, which Eric documents at the cureFFI.org blog. The paper reports […]
The ability to sequence DNA from a Guthrie card e.g. genomic or methylomic analysis (also see my post from last Summer) makes their more routine use in cancer genomics studies attractive. An area that would be great to see developed is the extraction of the […]
Saturday, February 13, 2016 14:45Def Leppard are playing “Pour some sugar on me” while we wait for Nick Loman to walk on stage – not sure if this was intentional.Nick Loman, University of Birmingham “Real-time genome sequencing in the fieldâ€. Four years since Clive’s 2012 […]
Saturday, February 13, 2016 12:12:Ross Wadsworth, KAPA Biosystems “Hyper Drive: high performance, streamlined workflows for RNA-seq and target enrichmentâ€. KAPA Hyper (Covaris frag, fast efficient library prep), KAPA Hyper+ (enzymatic frag, fast efficient library prep), now RNA is getting the Kapa touch!The RNA-seq workflow: mRNA […]
