Saturday, February 13, 2016 14:45Def Leppard are playing “Pour some sugar on me” while we wait for Nick Loman to walk on stage – not sure if this was intentional.Nick Loman, University of Birmingham “Real-time genome sequencing in the fieldâ€. Four years since Clive’s 2012 […]
Saturday, February 13, 2016 12:12:Ross Wadsworth, KAPA Biosystems “Hyper Drive: high performance, streamlined workflows for RNA-seq and target enrichmentâ€. KAPA Hyper (Covaris frag, fast efficient library prep), KAPA Hyper+ (enzymatic frag, fast efficient library prep), now RNA is getting the Kapa touch!The RNA-seq workflow: mRNA […]
Saturday, February 13, 2016 11:47:Joe Beecham Nanostring “New optical barcode chemistry’s power 3D biology & sequencing-simultaneous DNA, RNA, & protein analysis and low error-rate targeted sequencing†A sequencing system with no library prep or amplification – this is a pretty cool project. Joe is presenting […]
Saturday, February 13, 2016 11:26:Cynthia Hendrickson, Directed Genomics New England BioLabs “A novel fully customizable hybridization-based enrichment technology integrating capture and library preparation.†Cynthia was describing the new target capture chemistry she’s been building with NEB. I wrote up an overview of their method in […]
Friday, February 12, 2016 21:10: I’m flitting between sessions tonight…first up is a talk by a users of my lab, we generated the data for several hundred C.eleganss genomes using Nextera and HiSeq 2500 – not quite a 1000 genome project yet! Geoffrey Nelson Geoffrey Nelson, […]
Contrary to what you might think this post is not going to be about the sequencing technology or data being presented at AGBT this year, but what could have happened several years ago and has come to AGBT16 – real-time sequencing.In his minoTaur software demo […]
Jason BielasJason Bielas, Fred Hutchinson Cancer Research Center “Deep profiling of complex cell populations using scalable single cell gene expression analysisâ€. Early adopter of 10X single-cell RNa-seq platform – find out more at the 10X workshop this afternoon. Initially running experiments on PBMCs and finding […]
Anne WojcickiAnne Wojcicki, 23andMe “Making discoveries on the 23andMe platformâ€. Anne is going to focus on the benefit to the consumer of the Human genome. How will we decipher it, how will we cope with massive studies i.e. tens of millions of active participants.The 23andMe […]
Friday, February 12, 2016 09:40 (updated to improve the description of the chemistry and applications)Jay Flatley, Illumina “Beyond the $1000 Genome-What’s next for NGS?â€. He spent his time describing some of the key ingredients that have driven the sequencing tech at Illumina, and gave us […]
Thursday, February 11, 2016 21:35Mariateresa de CesareMariateresa de Cesare, University of Oxford “Unlocking the heterogeneity of the human transcriptome utilizing the ONT MinION TMâ€. The lab has been working with the ONT MinION for about two years. Mariateresa presented some of the Pros of RNA-seq, […]
