Our MiSeq has just been replaced after some performance issues and we are now getting ready to start running the upgraded version with the newest chemistry. Hopefully we’ll be getting the 10Gb+ that Illumina reported some users are achieving.In a GenomeWeb article yesterday Illumina responded […]
Many groups are working on next-generation sequencing tests for mutation profiling of tumours. There are two main approaches, amplicon sequencing (reviewed in a previous post) and in-solution capture capture (reviewed here and here); both of which only look at DNA which is not immediately limiting, […]
We have a tradition in the lab that you bring cakes in on your birthday. Home-made is always encouraged and one of my favourite party bakes is this Millionaire’s shortbread. Enjoy.Mmmm, sweeet!!!Make shortbread:200g/7oz butter115g/4oz caster sugar285g/10oz plain flourCream the butter and sugar, beat till fluffy, […]
In a few years hopefully every cancer patient in the UK will be screened for the most common somatic mutations. CRUK’s Stratified Medicine Project has already tested over 5000 patients as part of a program to roll-out more uniform testing in the NHS.There has been […]
The deadline for submitting your registration and abstract to ABGT closes tonight! As I said a few weeks ago this year the organisers are trying harder than ever to make sure the meeting reaches as wide an audience as possible. There are always grumblings from […]
I finally saw an image of a 2500 flowcell so you can stop looking at the one I mocked up based on discussions with Illumina personnel just before AGBT.One thing immediately struck me and that was how much a HiSeq 2500 flowcell looks like an […]
PCR was invented way back in 1985 and the first thermo-cyclers were released in Before this PCRs were done in tubes placed in separate water-baths. Polymerase was also added at each new cycle until someone worked out that Taq (discovered in 1976 by University of […]
Design, replication, multiplexing. These are the three things I spend most of my time discussing in experimental design meetings. In my institute we hold three 30-minute design sessions every week where users talk to my group and our bioinformatics core about how best to run a […]
George Church is one of the “godfathers of genomicsâ€*. In one of his latest publications, Next-Generation Digital Information Storage in DNA he demonstrates how to use DNA as an information storage medium. He’s not the first to do this and the supplemental information to the […]
This is a slightly wacky post but I did warn you I’d go off on tangents sometimes!My new bike, multi-tool comparisons and “how fast can you unfold a Brompton?”My old bike was stolen back in March. We had gone to the campus bar to celebrate […]
