Everyone knows BGI bought Complete Genomics last year. What is less clear is what BGI’s plans are for Complete’s platform (a little more at the bottom of this thread). There has also been a bit of buzz about seqeuncers being developed by the genomics institute […]
Our HiSeq 2500 v4 validation runs are just about to finish and I thought I’d share some details. Ideally I’d give you access to the runs so you can dig around yourselves but until Illumina makes this possible on a per lane basis in BaseSpace […]
I’ve been accused of being both too sceptical and too glowing about Illumina’s technology over the years I’ve been writing this blog. It very much depends on who you talk to and which post you might have just read. Believe it or not I don’t […]
Pooling samples for multiplexed sequencing has become the norm for many researchers, especially in the light of 1TB sequencing in 6 days! However many users struggle to get pools nicely balanced. I’ve just seen a tool I think is helpful so I thought I’d share […]
I had a chat with a colleague about the insanity of the Cambridge property market and determined that a house here could cost more than the 1000 genomes project. In theory this would cost $1,000,000 if run on HiSeq X Ten today and only take […]
We just had a shiny new NextSeq installed and the validation run gave 480M reads (about 20% above the spec of >400M reads) and 83.1%Q30 (against Illumina’s spec of >75%Q30 at 2×150bp). But the initial reaction internally was that the error rates looked too high […]
HiSeq rapid is going to be getting paired-end 250bp reads. Assuming you can sequence with a 500bp insert (+/-50bp) then we can expect to get perhaps 200-300M pairs of overlapping reads that can be merged to create 200-300M 500bp sequences. I suspect this is going […]
A wonderful story appeared on BBC news website today about the company Planet Labs who are using a similar idea Illumina implemented in designing NextSeq – borrow from consumer electronics to advance science. Rather than building sequencers Planet Labs have built and launched a flock […]
I’ve just returned from Illumina’s 2014 scientific summit in Prague which was a great meeting with loads of interesting speakers, great food (check out this restaurant if you’re ever in Prague) and lots of opportunity for engagement with other users as well as Illumina executives. […]
DMSO is used in many protocols as a ‘universal solvent’, it’s great for solublising small molecules and you’ve almost certainly used DMSO when freezing cells or in PCR. Unfortunately it’s relative ubiquity of use in research labs means it’s negative impacts on some compounds can […]
