We’ve all been there: PubMed returns over 2500 RNA-seq papers, and there’s still 800 left when you only search the title! How do you find the best papers to read? PubMed can help a little more with your quest to find out more about RNA-seq […]
AGBT had lots of presentations on both clinical and single-cell work last February and on Wednesday, February 12th Aviv Regev, of the Broad Institute described her groups experience with the Fluidigm C1 system, and their early work in understanding cell-to-cell communication in the immune system. […]
How may days till Christmas…Dear Santa, I know this might be a little early but I thought I’d start my Christmas list. My presents came a little late last year, and there was nothing under the tree on Christmas morning (you should’ve heard me crying). […]
Everyone knows BGI bought Complete Genomics last year. What is less clear is what BGI’s plans are for Complete’s platform (a little more at the bottom of this thread). There has also been a bit of buzz about seqeuncers being developed by the genomics institute […]
Our HiSeq 2500 v4 validation runs are just about to finish and I thought I’d share some details. Ideally I’d give you access to the runs so you can dig around yourselves but until Illumina makes this possible on a per lane basis in BaseSpace […]
I’ve been accused of being both too sceptical and too glowing about Illumina’s technology over the years I’ve been writing this blog. It very much depends on who you talk to and which post you might have just read. Believe it or not I don’t […]
Pooling samples for multiplexed sequencing has become the norm for many researchers, especially in the light of 1TB sequencing in 6 days! However many users struggle to get pools nicely balanced. I’ve just seen a tool I think is helpful so I thought I’d share […]
I had a chat with a colleague about the insanity of the Cambridge property market and determined that a house here could cost more than the 1000 genomes project. In theory this would cost $1,000,000 if run on HiSeq X Ten today and only take […]
We just had a shiny new NextSeq installed and the validation run gave 480M reads (about 20% above the spec of >400M reads) and 83.1%Q30 (against Illumina’s spec of >75%Q30 at 2×150bp). But the initial reaction internally was that the error rates looked too high […]
HiSeq rapid is going to be getting paired-end 250bp reads. Assuming you can sequence with a 500bp insert (+/-50bp) then we can expect to get perhaps 200-300M pairs of overlapping reads that can be merged to create 200-300M 500bp sequences. I suspect this is going […]
