Illumina recently released a new version of HCS v2.2.38 for the HiSeq. The update improves cluster definition significantly and enables low-diversity sequencing. It’s a great update and one that’s making a big impact on a couple of projects here. Better RRBS: the current RRBS protocols […]
Illumina recently released a new version of HCS v2.2.38 for the HiSeq. The update improves cluster definition significantly and enables low-diversity sequencing. It’s a great update and one that’s making a big impact on a couple of projects here. Better RRBS: the current RRBS protocols […]
I’ve been working with microarrays since 2000 and ever since RNA-seq came on the scene the writing has been on the wall. RNA-seq has so many advantages over arrays that we’ve been recommending them as the best way to generate differential gene expression data for […]
FFPE makes DNA extraction difficult; DNA yields are generally low, quality can be affected by fixation artefacts and the number of amplifiable copies of DNA are reduced by strand-breaks and other DNA damage. Add on top of this almost no standardisation in the protocols used […]
It strikes me that if you can now sequence a genome for $1000, then you could buy 1000x coverage for not much more than a 30x genome cost a couple of years ago! Using a PCR-free approach I can imagine that this would be the […]
A comment popped up on a post I’d written in April last year “MiSeq (and 2500) owners better read this and beware”that made me think I’d ask readers the question in the title: “how many of you are using the MiSeq bleach wash protocol? online […]
With the BGI aiming to get their own diagnostic sequencing tests on the market, and the purchase and development of Complete Genomics technology – Omega, a question that could be asked is whether BGI will ever stop using Illumina technology?BGI are still the largest install […]
I’ve been a user of Altmetric for a while now and very much like what they are doing with article metrics. I’m sure many Core Genomics readers will also have seen the Altmetric badge on their own papers. Now Altmetric are hosting their first conference.The […]
GWAS is still in the news and still finding hits, the number of GWAS hits has increased rapidly since the first publication for AMD in 2005. Watch the movie to see the last decade of work!A recent paper in PNAS seems to have got people […]
Personally I’m thinking that the aluminium plate might make a pretty nifty bottle opener!
