In my last post I wrote about a computational screen of TCGA data and its use in repurposing approved drugs and/or finding new drug candidates for cancer patients. The work demonstrated the possibilities for finding novel treatments, but I also pointed to a cautionary Vemurafenib […]
A fantastic paper just out in Cancer Cell: In Silico Prescription of Anticancer Drugs to Cohorts of 28 Tumor Types Reveals Targeting Opportunities from Nuria Lopez-Bigas’s BioMedical Genomics lab in Barcelona.They have developed an in silico prescription strategy by identifying the driver events in TCGA […]
Another fun year at AGBT in sunny Marco Island. There has been some good blog coverage this year, but not from me; I’ve been Tweeting instead @CIgenomics on #AGBT15, see what happened on Twitter.The UCSC Twitter trackBlog coverage: GenomeWeb were there for those who could […]
The meeting kicks off today with Illumina’s user meeting and I’m going to give “Tweeting the meeting” a go this year. Keep an eye on @CIgenomics and #AGBT15James.
We’ll soon be back in sunny Florida (current forecast is low to mid 20’s) for another cram packed Advances in Genome & Biotechnology meeting. Of course the focus is still on sequencing, but with instruments like to CyTof coming along and huge improvements in total […]
A recent BioTechniues practical guide: Libraryconstruction for next-generation sequencing: Overviews and challenges, written by Steven Head et al from the The ScrippsResearch Institute NGS and Microarray Core Facility; might be just the thing to hand out to your NGS newbies as it covers pretty much […]
Cellular Research released their latest development: Resolve for single cell mRNA-seq sample-prep at under £1 per cell. A paper in today’s Science describes the method: Combinatorial labeling of single cells for gene expression cytometry. Using CytoSeq (why not Cyto-seq) 10,000 or 100,000 cells can be […]
Updated: The cost per M reads and cost per Gb figures in the original posting were wrong – damned Excel operator error! I’ve fixed them, again. Thanks to Shawn for his comments.I’ve been asked about the difference between the Illumina sequencer line-up so many times […]
The amount of RNA used in an RNA-seq library prep is often listed as a competitive advantage by kit manufacturers. As late as 2004 I used up to 30ug of RNA in a microarray prep, and even a couple of years ago 100ng was considered […]
