A year ago I surveyed the costs of Illumina library prep and found over 15 providers; they offer generally the standard Illumina library prep method but with prices ranging from £15-£60 per sample. There was some real innovation amongst some of these kits too, enabling […]
I’m often trying to find an image for a post and it can be tough trying to find something that can be used freely. The U.S. DOE has an image gallery from the Genomic Science program, which includes archived images from the Human Genome Project. […]
Michael Snyder has a great paper published in AJRCCM: Exome Sequencing of Neonatal Blood Spots Identifies Genes Implicated in Bronchopulmonary Dysplasia (BPD). BPD is a lung disease of premature babies which appears to have a strong genetic component and was investigated using exome sequencing. The […]
Next-Generation DNA Sequencing Informatics (2nd edition), edited by Stuart M. Brown (blogger) is a book to get you well and truly started in NGS Bioinformatics. The twelve chapters cover QC, sequence alignment, assembly, transcriptome and ChIP-seq analysis, visualisation, and much else besides. For this review […]
6310 MinIONs fit inside one PacBIO!London Calling: No-one who is interested in NGS can have missed the developments from Oxford Nanopore, Twitter was on fire last week during the London Calling event and there are some great reviews of what went on for those of […]
The GIAB consortium (@GenomeInABottle) took a major step forward today when it released the first NIST reference material for Human genome sequencing, the story even made it into the New York Times. It comes at an important time when we’re moving into an era where […]
Oops, I forgot to publish this post after the meeting!So many posters at AGBT and so little time. I did not get round everything and these highlights were some of the many that caught my eye. I’d like to hope the organisers make the posters […]
The cost of sequencing has dropped precipitously over the last five years, the cost of library prep has not moved by anywhere near as much. For some experiments this is a major headache, particularly in small genomes, metagenomics, and for us, RNA-seq. Reducing the cost […]
One of our statisticians recently co-authored a paper in Nature Methods on the use and misues of p-values: The fickle P value generates irreproducible results. After reading this paper I really felt that I’d learned something useful; that p-values, which we use to determine if […]
This is sort of a book review: Statistics for Biologists is a collection of articles from the Nature journals including the Nat Meth Points of Significance column. This collection brings together the POS articles published since 2013, which aim to provide biologists with a basic […]
