Illumina have discontinued the version 1 of the NextSeq chemistry. Launched in January of last year the NextSeq was a revolutionary new sequencer, although not everyone was an immediate fan. The V2 chemistry was launched just before AGBT and the data certainly looked a lot […]
Illumina have a new offer on their Understand Your Genome (UYG) program that means you can get your genome sequenced, analysed and clinically interpreted for under £2000. Interested? Then there are a few requirements, mainly that you give informed consent and get a doctors prescription for […]
A paper just published in Nature Communications describes a molecular analysis of Pancreatic Cancer by tumour exome and ctDNA targeted sequencing. The results showed enrichment of mutations in known PaCa associated genes, and identified clinically actionable mutations in over 1/3rd of patients. Marc Sausen in […]
The use of the pre-print servers like the original arXiv and bioRxiv appears to be growing among some of the groups I follow. You’ve only got to read Jeff Leeks post about this and their Ballgown paper (published at NatBiotech) or Nick Loman’s or Casey […]
What do 48 replicates tell you about RNA-seq DGE analysis methods: that two the most widelyâ€used of the tools DESeq and edgeR are probably the best tools for the job*. These two tools also top the rankings of RNA-seq methods as assessed by citations with […]
Back in 2010 a HiSeq 2000 cost $690,000 – you’ll be lucky to get anything today. A V4 2500 might get you $100+k, but your V4 capable V3 just $20k. Depreciation sucks!In accountancy, depreciation refers to the decrease in value of an asset over time. […]
A paper on the BioArxiv describes a new metric to measure scientific journals by based on their efficiency of information distribution (citations) within the network of journals. It provides a providing together a complex picture of the intricate relations between scientific journals; but basically Science, […]
I realised I’d not covered the recent droplet sequencing papers (the post was sitting in my draft blogs pile) and wanted to make sure I did after posting on single-cell RNA-seq last week. The use of droplets has some benefits over microfluidics where the current […]
A team from Columbia University present a microfluidic device that can capture single-cell lysates in picotire plates and produce single-cell 3′ tag RNA-Seq library prep at $0.10 cents per cell: Scalable microfluidics for single cell RNA printing and sequencing. They discuss two methods – RNA […]
When George Church talks about something it often turns out to be a good idea to listen. He’s been talking about in situ sequencing for many years and the technology looks to be ready for take off. It could be a niche method used by […]
