Switch Mechanism at the 5′ End of RNA Templates Smart-Seq was developed as a single-cell sequencing protocol with improved read coverage across transcripts (Ramskold et al., 2012). Complete coverage across the genome allows the detection of alternative transcript isoforms and SNPs. There are 2 versions […]

Switch Mechanism at the 5′ End of RNA Templates Smart-Seq was developed as a single-cell sequencing protocol with improved read coverage across transcripts (Ramskold et al., 2012). Complete coverage across the genome allows the detection of alternative transcript isoforms and SNPs. There are 2 versions […]

Single-Cell Triple Omics Sequencing scTrio-Seq can analyze genomic CNVs, the DNA methylome, and the transcriptome of an individual mammalian cell simultaneousl. This approach is an extension of previous methods, such as scMT-seq (Hu et al., 2016)Advantages: Accurately analyzes the mechanism by which the transcriptome, genome, […]

Single-Cell mRNA Sequencing scRNA-Seq provides deeper insight to the multi-tiered complexity of different cells within the same tissue type (Tang et al., 2009). scRNA-Seq has now been adapted widely into other methods in the single-cell RNA sequencing field.In this method, single cells are isolated manually […]

Single-Cell RNA Barcoding and Sequencing SCRB-Seq is a cost-efficient, multiplexed, single-cell mRNA sequencing technique (Soumillon et al., 2014). SCRB-Seq isolates single cells into wells using FACS. After cell lysis, poly(A)+ mRNAs are annealed to a custom primer containing a poly(T) tract, UMI, well barcode, and […]

Single-Cell Methylome and Transcriptome Sequencing scM&T-Seq allows parallel analysis of both epigenetic and gene expression patterns from single cells using Smart-seq2 and scBS-seq (Angermueller et al., 2016). scM&T-Seq is built upon G&T-seq, but instead of using MDA for DNA sequencing, it uses scBS-Seq to determine […]

Whole-Transcript Amplification for Single Cells The Quartz-Seq method optimizes whole-transcript amplification (WTA) of single cells (Sasagawa et al., 2013). In this method, an RT primer with a T7 promoter and PCR target is first added to the extracted mRNA. RT synthesizes first-strand cDNA, after which […]

Peptide Nucleic Acid (PNA)-Assisted Identification of RNA Binding Proteins PAIR uses PNAs to capture RBPs in vivo (Zielinski et al., 2006) (Zeng et al., 2006). The PNAs are coupled to a cell membrane-penetrating peptide (CPP) to deliver PNAs efficiently into living cells, as well as […]

A Single-Nucleus RNA-Seq from Frozen Tissues Nuc-Seq is an RNA sequencing technique optimized for isolating and sequencing nuclear RNA from frozen tissue samples (Krishnaswami et al., 2016). Nuc-Seq avoids proteolytic treatment during nuclei dissociation from cells, minimizing gene expression changes resulting from common protease dissociation […]

Massively Parallel RNA Single-Cell Sequencing Framework MARS-Seq profiles the transcriptional dynamics of single cells in an automated and massively parallel workflow with high resolution (Jaitin et al., 2014). MARS-Seq can be used with in vivo samples containing a wide variety of different cell subpopulations.Single cells […]