Single-Cell mRNA Sequencing

scRNA-Seq provides deeper insight to the multi-tiered complexity of different cells within the same tissue type (Tang et al., 2009). scRNA-Seq has now been adapted widely into other methods in the single-cell RNA sequencing field.In this method, single cells are isolated manually under a microscope and lysed. Next, mRNAs are purified and primed with a poly(T) primer for reverse transcription. Unreactive primers are removed by exonuclease I digestion. Poly(A) tails are added to the first strand cDNA at the 3′ end and annealed to poly(T) primers for second-strand cDNA generation. Finally, the cDNAs are PCR-amplified, sheared, and prepared into sequencing libraries.


  • Single-cell_resolution transcriptomic analysis
  • Able to detect unknown splice junctions


  • Very low throughput
  • No molecular barcodes used


Illumina Library prep and Array Kit Selector


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