Single-cell sequencing is making waves with many exciting developments over the last couple of years. Hardware is available from the companies like 10X Genomics, Fluidigm, Wafergen, BioRad (see GenomeWeb), RainDance (see GenomeWeb), Dolomite Bio. Chemistry for RNA and Genome sequencing such as SMART-Seq V4 and TruPrime, opens up all sorts […]
The latest round of fundraising by ONT brought in over $100 million, that makes a total of around $X million invested over the last X years. This round of funding was led by GT Healthcare, a Hong Kong based investment group. According to the Financial Times […]
Qiagen are now finally selling the GeneReader after a hiatus of several years. This was caused by issues getting a working instrument out of the original IBS technology, and also held, temporarily, up by recent Illumina litigation. GenomeWeb covered the relaunch back in November. Qiagens updated […]
A couple of Tweets today point to the amazing lengths Oxford Nanopores MinION sequencer is capable of generating – over 400kb! Dominik Handler Tweeted a plot showing read distribution from a run . In replies following the Tweet he describes the DNA handling as […]
The UK recently approved an NIPT test to screen high risk pregnancies for foetal trisomy 21, 13, or 18 after the current primary screening test, and in place of amniocentesis (following on from the results of the RAPID study). I am 100% in favour of […]
Illumina stock dropped 25% after a hammering by the stock market with their recent announcements that Q3 revenues would be 3.4% lower than expected at just $607 million. This makes Illumina a much more attractive acquisition (although I doubt this summers rumours of a Thermo […]
The use of DNA methylation analysis by NGS has become a standard tool in many labs. In a project design discussion we had today somebody mentioned the use of a control for bisulfite conversion efficiency that I’d missed, as its such a simple one I […]
This article was commissioned by Lexogen GmbH. My lab has been performing RNA-seq for many years, and is currently building new services around single-cell RNA-seq. Fluidigm’s C1, academic efforts such as Drop-seq and inDrop, and commercial platforms from 10X Genomics, Dolomite Bio, Wafergen, Illumina/BioRad, RainDance and others makes establishing […]
At the recent Wellcome Trust conference on Single Cell Genomics (Twitter #scgen16) there was a great talk (her slides are online) from Stephanie Hicks in the @irrizarry group (Department of Biostatistics and Computational Biology at Dana-Farber Cancer Institute). Stephanie was talking about the recent work she’s […]
DeciBio have a great interactive Tableau dashboard which you can use to browse and filter their analysis of 97 “laboratory biomarker analysis†ImmunOncolgy clinical trials; see: Diagnostic Biomarkers for Cancer Immunotherapy – Moving Beyond PD-L1. The raw data comes from ClinicalTrials.gov where you can specify […]
