In this post I describe an idea for using SNP fingerprinting for single-cell RNA-Seq to identify which sample each individual cell comes from in a multiplexed library prep. The reason for my thinking about this is that single-cell experiments are expensive. Although the cost-per-cell can […]
We do lots of DNA, RNA and NGS library normalisations mostly by hand after calculating dilutions in Excel. It works well but is cumbersome and prone to error. We’re fortunate to have an Agilent Bravo robot and are now automating more of this with excellent […]
Today I was given an exclusive preview from the newest nanopore sequencing company on the planet, Norfolk Nanopore Technology. The new “Polonopore”technology has been incubating in the Norwich Research Park BioIncubator under the same roof as TGAC. NNT’s brand spanking new nanopore sequencer could mean […]
De novo assembly of complex genomes is still harder than many would like.For Cancer genomes de novo assembly could be the ultimate method for discovery of all somatic events. But the analysis requires long-reads or long-insert reads and this generally eats up lots of DNA […]
I like encouraging people to try to come up with new ideas. One day I want to add ‘inventor’ to my CV and get a patent, I posted a while ago about an idea that came very close but was pipped to the post by […]
One of the things I most like about my job is being allowed to think. NGS has provided a very fertile ground for innovation and lots of novel ideas have become products we use regularly today. It is sometimes to easy to forget that any […]
