Chromatin Conformation Capture Sequencing 3C-Seq (Duan et al., 2012), Capture-C, and Hi-C (Lieberman-Aiden et al., 2009) comprise a family of methods for analyzing chromatin interactions. Capture-C adds an additional pull-down of the biotinylated fragments with magnetic beads to the 3C method. A new refinement of […]

All of the DNA Protein Interaction sequencing methods are collected together under this heading

Map DNA Single-Strand Breaks SSB-Seq maps in vivo DNA single-strand breaks (SSBs) on a genome-wide scale (Baranello et al., 2014).In this method, gDNA is isolated and subjected to nick-translation by DNA polymerase I in the presence of deoxynucleotide triphosphates (dNTPs), including digoxigenin-labeled dUTP, and a […]

Double-Stranded Break Labeling Break-seq combines DSB labeling with NGS to map chromosome breaks with improved sensitivity and resolution (Hoffman et al., 2015). DNA trapped in agarose plugs is end-repaired and labeled with biotin. The agarose is then digested by _-agarase, followed by dilution and fragmentation. […]

All of the DNA Break Mapping methods are collected together under this heading

All of the DNA sequencing methods are collected together under this heading