17 04, 2013

The 8 rules of cake club

By | April 17th, 2013|Categories: Uncategorized|1 Comment

1st RULE: Everyone votes at cake club. 2nd RULE: EVERYONE votes at cake club. 3rd RULE: If someone says “burnt” the cake is over. 4th RULE: Only one person can bake. 5th RULE: Only one cake at a time. 6th RULE: No shop bought, no […]

15 04, 2013

MiSeq (and 2500) owners better read this and beware

By | April 15th, 2013|Categories: Uncategorized|3 Comments

Update 25th April: Illumina have released a support bulletin “Best Practices for High Sensitivity Applications: Minimizing Sample Carryover”, this is accessible through Illumina’s website, or SEQanswers. You’ll need an Illumina login!Illumina say that sample carryover is more likely to have an effect on very low […]

5 04, 2013

eBay as a way to equip your lab…

By | April 5th, 2013|Categories: Uncategorized|2 Comments

We have some old lab equipment we are getting rid of much of it will be recycled into other University departments. Some will be sold to equipment resellers like Biagen, Alliance Analytical, and Harlow Scientific. Some of it may even end up in the skip. […]

28 03, 2013

Life Tech beats Illumina to the starting gates

By | March 28th, 2013|Categories: Uncategorized|1 Comment

Updated after reading this post on GenomeWeb!Oxford University and the NHS announced the UK’s first next-generation sequencing based test for Cancer. The test uses Life Technologies Ion Torrent PGM and AmpliSeq to assay 46 gene “hotspots”, but should increase to around 150 genes in the […]

26 03, 2013

Imputation of LOH from 1x genome sequence

By | March 26th, 2013|Categories: Uncategorized|0 Comments

I would like to move from microarray based genotyping to NGS for copy-number and loss-of-heterozygosity analysis. The copy-number bit should be relatively simple and I hope we’ll have done this by the end of the year, however the LOH analysis is more complex as it […]

25 03, 2013

Making NGS greener

By | March 25th, 2013|Categories: Uncategorized|4 Comments

Does your lab look like this? We get most of our deliveries on dry-ice shipped from European distribution centres. All the polystyrene and dry-ice are the tip of our energy consumption iceberg. Genomics sciences have as much environmental impact as just about everything else, and […]

14 02, 2013

AGBT… where are the big announcements

By | February 14th, 2013|Categories: Uncategorized|0 Comments

The 14th AGBT meeting kicks off in one week. Unfortunately I won’t be there this year so don’t expect a daily round-up of what’s good from me! For those of you blogging and tweeting AGBT have crafted some simple graphics to indicate what you can, […]

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