1 02, 2017

Single-cell copy number analysis with SCI-Seq

By | February 1st, 2017|Categories: Next-generation sequencing, Single-cell sequencing|0 Comments

Andrew Adey’s group at the Department of Molecular & Medical Genetics in Oregon Health & Science University published SCI-seq in Nature Methods earlier this week (it has been on the BioRxiv since July). SCI-Seq is a method for single-cell copy number analysis that is likely […]

15 12, 2016

The 5 hardest things about single-cell sequencing

By | December 15th, 2016|Categories: Next-generation sequencing, Single-cell sequencing|2 Comments

Single-cell sequencing is making waves with many exciting developments over the last couple of years. Hardware is available from the companies like 10X Genomics, Fluidigm, Wafergen, BioRad (see GenomeWeb), RainDance (see GenomeWeb), Dolomite Bio. Chemistry for RNA and Genome sequencing such as SMART-Seq V4 and TruPrime, opens up all sorts […]

17 10, 2016

SIRVs: RNA-seq controls from @Lexogen

By | October 17th, 2016|Categories: Commissioned content, Next-generation sequencing, Single-cell sequencing|2 Comments

This article was commissioned by Lexogen GmbH. My lab has been performing RNA-seq for many years, and is currently building new services around single-cell RNA-seq. Fluidigm’s C1, academic efforts such as Drop-seq and inDrop, and commercial platforms from 10X Genomics, Dolomite Bio, Wafergen, Illumina/BioRad, RainDance and others makes establishing […]

16 09, 2016

Reporting on Fluidigm’s single-cell user meeting at the Sanger Institute

By | September 16th, 2016|Categories: Conferences, Next-generation sequencing, Single-cell sequencing|0 Comments

The Genomics community is pushing ahead fast on single-cell analysis methods as these are revolutionising how we approach biological questions. Unfortunately my registration went in too late for the meeting running at the Sanger Institute this week (Follow #SCG16 on Twitter), but the Fluidigm pre-meeting was a […]

28 07, 2016

10X Genomics single-cell 3’mRNA-seq explained

By | July 28th, 2016|Categories: 10X Genomics, Next-generation sequencing, Single-cell sequencing|2 Comments

10X Genomics have been very successful in developing their gel-bead droplet technology for phased genome sequencing and more recently, single-cell 3’mRNA-seq. I’ve posted about their technology before (at AGBT2016, and March and November 2015) and based most of what I’ve written on discussion with 10X […]

12 02, 2016

AGBT16 10X Genomics workshop

By | February 12th, 2016|Categories: 10X Genomics, Single-cell sequencing|0 Comments

10X Genomics were one of the stars of the show at AGBT15, this year they’re bowling attendees over with new developments. They’ve obviously had an awfully busy year. They announced lots at JP Morgan (GenomeWeb, Core-Genomics). I got a sneak peak (as did Keith at […]

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