At our recent institute symposium I added an additional graphic to try and get people thinking about how much their sequencing costs.
Can you work it out: Below are six pairs of circles representing genome, methylome, RNA-seq (GX), ChIP-seq, Exomes and amplicons, size is proportional to cost and the two colours represent library preparation and sequencing.
- Can you determine which application is which?
- Which colour is library prep?
- Which colour is sequencing?
- How much do you think each application costs?
Answers in the form of comments or drop me an email. The results here surprised me, I thought people had a better idea of the actual costs but it turns out people have some pretty wild ideas!
Very nice post. Working on the analysis! 🙂
My guess:
1) ChIP-Seq
2) RNA-Seq
3) Amplicon
4) Exomes
5) Genome
6) Methylome
Orange = prep; Red = sequencing
Interested in seeing the results! Hope they aren't too far off – I only assist with the bioinformatic analysis!
My guess:
orange is sequencing (pricing is similar for all library prep methods), red is library prep
1) genome
2) RNA-seq
3) exome
4) methylome
5) CHiP-Seq
6) Amplicon
My estimation is based on a pricing per lane sequencing.
……?
This is a fun game.
In the order of my logic:
#6 is Methylome, not presumably RRBS (Reduced Representation Bisulfite) but rather Whole Human, library ~$120, seq ~$5500 (~120GB seq)
#5 is Whole Genome (~90GB seq), library ~$100, seq ~$4000
#2 is Whole Exome (~5GB seq), library ~$40, seq ~$250
#1 is ChIP, 5M tags, library prep ~$110, seq ~$150
#4 is Gene Expression RNA-Seq, 3M tags, library prep ~$130, seq $90
Can you determine which application is which?
1-Genome
2-Exome
3-Amplicon Sequencing
4-RnaSeq
5-Methylome
6-ChipSeq
Which colour is library prep?
Red
Which colour is sequencing?
Orange
How much do you think each application costs?
Library prep = Red
Sequencing = Orange
1 = Genome
2 = RNAseq
3 = Amplicon
4 = Exome
5 = Methylome
6 = ChIPseq
This is our logic guess as analysis guys not knowing the current prices nor if the cost is per sample, per depth or per lane.
Library prep = Red
Sequencing = Orange (assuming constant cost for sequencing a whole lane with one individual)
1: RNAseq
2: Exome
3: Amplicon
4: Genome
5: ChIPseq
6: Methylome
Or probably something totally different…
Not sure about exome/ChIPseq.
By the way, it would be really cool if you could add a blue circle with bioinformatics analysis cost.
fun game!
yellow=Seq, red = Prep
1- genome
2 – exome
3- amplicons
4- RNA seq
5- CHIP seq
6 – methylome
?