m6A-Specific Methylated RNA Immunoprecipitation Sequencing
MeRIP-Seq maps m6A-methylated RNA. In this method, m6A-specific antibodies are used to immunoprecipitate RNA. RNA is reverse-transcribed to cDNA and sequenced. Deep sequencing provides high-resolution reads of m6A-methylated RNA (Meyer et al., 2012)
Similar methods: miCLIP, m6A-LAIC-Seq
- Maps m6A-methylated RNA
- Antibodies not specific to the target will precipitate nonspecific RNA modifications.
Illumina Library prep and Array Kit Selector
Bian Q. and Cahan P. Computational Tools for Stem Cell Biology. Trends Biotechnol. 2016;
Gokhale N. S., McIntyre A. B., McFadden M. J., et al. N6-Methyladenosine in Flaviviridae Viral RNA Genomes Regulates Infection. Cell Host Microbe. 2016;