SMDB
Single-Molecule Droplet Barcoding
SMDB is a method to leverage short-read sequencing to obtain long and accurate reads (Lan et al., 2016). Using a microfluidics system, the method isolates, amplifies, fragments, and barcodes single DNA molecules in aqueous picoliter droplets. This approach allows the full-length molecules to be sequenced with multi-fold coverage, using short-read sequencing.
Advantages:
- Limit of detection scales with the number of molecules sequenced and can be orders of magnitude more sensitive than conventional NGS
Disadvantages:
- Requires specialized hardware
Reagents:
Illumina Library prep and Array Kit Selector
Reviews:
None available yet
References:
Lan F., Haliburton J. R., Yuan A. and Abate A. R. Droplet barcoding for massively parallel single-molecule deep sequencing. Nat Commun. 2016;7:11784