UMI-4C

Circular Chromosome Conformation Capture with Unique Molecular Identifiers

This variation on the 4C approach uses UMIs to derive high-complexity quantitative chromosome contact profiles with controlled signal-to-noise ratios (Schwartzman et al., 2016). It is an efficient and accurate method for analyzing targeted loci. The method is paired with software to analyze the data (https://bitbucket.org/tanaylab/umi4cpackage).

Advantages:

  • Improved sensitivity and specificity
  • Multiplexing allows robust comparison of contact distributions between loci and conditions

Disadvantages:

  • None reported


Reagents:

Illumina Library prep and Array Kit Selector



Reviews:

None available yet

References:

Schwartzman O., Mukamel Z., Oded-Elkayam N., et al. UMI-4C for quantitative and targeted chromosomal contact profiling. Nat Methods. 2016;13:685-691