Circular Chromosome Conformation Capture with Unique Molecular Identifiers
This variation on the 4C approach uses UMIs to derive high-complexity quantitative chromosome contact profiles with controlled signal-to-noise ratios (Schwartzman et al., 2016). It is an efficient and accurate method for analyzing targeted loci. The method is paired with software to analyze the data (https://bitbucket.org/tanaylab/umi4cpackage).
- Improved sensitivity and specificity
- Multiplexing allows robust comparison of contact distributions between loci and conditions
- None reported
Illumina Library prep and Array Kit Selector
None available yet
Schwartzman O., Mukamel Z., Oded-Elkayam N., et al. UMI-4C for quantitative and targeted chromosomal contact profiling. Nat Methods. 2016;13:685-691