Cap Analysis Gene Expression Sequencing

CAGE measures RNA expression and maps TSS in promoters (Takahashi et al., 2012). In this method, RNA is first reverse-transcribed using random primers. The RNA cap and 3′ ends are biotinylated. Nonhybridized, single-stranded RNAs are digested with RNase, leaving 5′ complete cDNAs that are captured using streptavidin beads. The cDNA is processed for sequencing.


  • Measures RNA expression levels and maps TSS in promoter regions
  • Provides precise mapping of TSS with single-nucleotide resolution



Illumina Library prep and Array Kit Selector


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