CAP-seq

CXXC Affinity Purification Sequencing

CAP-seq maps methylated CpGs in gDNA (Illingworth et al., 2010). In this method, DNA is incubated with CXXC bound to nickel-charged sepharose beads. Next, DNA is eluted from the complex and sequenced. Deep sequencing provides insights into methylated CpG sites in gDNA.

Advantages:

  • Targets CpG islands

Disadvantages:

  • None known


Reagents:

Illumina Library prep and Array Kit Selector



Reviews:

None available yet

References:

Vilborg A., Passarelli M. C., Yario T. A., Tycowski K. T. and Steitz J. A. Widespread Inducible Transcription Downstream of Human Genes. Mol Cell. 2015;59:449-461

Smallwood S. A., Lee H. J., Angermueller C., et al. Single-cell genome-wide bisulfite sequencing for assessing epigenetic heterogeneity. Nat Methods. 2014;11:817-820

Suzuki M. M., Yoshinari A., Obara M., et al. Identical sets of methylated and nonmethylated genes in Ciona intestinalis sperm and muscle cells. Epigenetics Chromatin. 2013;6:38